Detailed information of TA system TA203489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31714..32444
• Replicon: chromosome
• Accession: NZ_CP075345
• Organism: Enterobacter kobei strain S18_CRE18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J0PHU0
• Locus tag: KJE18_RS00150
• Protein ID: WP_014881928.1
• Coordinates: 31714..32028 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KJE18_RS00155
• Protein ID: WP_023331687.1
• Coordinates: 32025..32444 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJE18_RS00130 (KJE18_00130)	27379..28467	+	1089	WP_014881924.1	cellulase family glycosylhydrolase	-
KJE18_RS00135 (KJE18_00135)	28370..29554	-	1185	WP_014881925.1	multidrug efflux MFS transporter EmrD	-
KJE18_RS00140 (KJE18_00140)	29730..30563	-	834	WP_014881926.1	EamA family transporter	-
KJE18_RS00145 (KJE18_00145)	30626..31072	-	447	WP_014881927.1	GNAT family N-acetyltransferase	-
KJE18_RS00150 (KJE18_00155)	31714..32028	+	315	WP_014881928.1	type II toxin-antitoxin system HigB family toxin	Toxin
KJE18_RS00155 (KJE18_00160)	32025..32444	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
KJE18_RS00160 (KJE18_00165)	32594..32692	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
KJE18_RS00165 (KJE18_00170)	32799..34487	+	1689	WP_014881930.1	acetolactate synthase large subunit	-
KJE18_RS00170 (KJE18_00175)	34491..34778	+	288	WP_010426528.1	acetolactate synthase small subunit	-
KJE18_RS00175 (KJE18_00180)	34861..35454	+	594	WP_014881931.1	transcriptional regulator UhpA	-
KJE18_RS00180 (KJE18_00185)	35451..36955	+	1505	Protein_35	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12437.60 Da
• Isoelectric Point: 10.2825

>T203489 WP_014881928.1 NZ_CP075345:31714-32028 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCPTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T203489 NZ_CP100102:c2530260-2529343 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCATCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT203489 WP_023331687.1 NZ_CP075345:32025-32444 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT203489 NZ_CP100102:c2531024-2530266 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A2J0PHU0