Detailed information of TA system TA020348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 283929..284468
• Replicon: chromosome
• Accession: NC_002695
• Organism: Escherichia coli O157:H7 str. Sakai

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: ECs_0252
• Protein ID: NP_308279.1
• Coordinates: 283929..284189 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: ECs_0253
• Protein ID: NP_308280.1
• Coordinates: 284208..284468 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECs_0249	281612..282190	+	579	NP_308276.1	D-sedoheptulose 7-phosphate isomerase	-
ECs_0250	282295..283062	+	768	NP_308277.1	type 2 glutamine amidotransferase family protein	-
ECs_0251	283033..283773	-	741	NP_308278.1	L,D-transpeptidase-related protein	-
ECs_0252	283929..284189	-	261	NP_308279.1	predicted toxin of the YafQ-DinJ toxin-antitoxin system	Toxin
ECs_0253	284208..284468	-	261	NP_308280.1	antitoxin of YafQ-DinJ toxin-antitoxin system	Antitoxin
ECs_0254	284654..285427	+	774	NP_308281.2	endopeptidase	-
ECs_0256	286245..287984	-	1740	NP_308284.2	flagellar export pore protein	-
ECs_0257	288079..288714	+	636	NP_308283.2	flagellar system protein MbhA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T20348 NP_308279.1 NC_002695:c284189-283929 [Escherichia coli O157:H7 str. Sakai]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T20348 NC_002695:c284189-283929 [Escherichia coli O157:H7 str. Sakai]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT20348 NP_308280.1 NC_002695:c284468-284208 [Escherichia coli O157:H7 str. Sakai]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT20348 NC_002695:c284468-284208 [Escherichia coli O157:H7 str. Sakai]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA