Detailed information of TA system TA203468

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrE-as-bsrE/-
• Location: 2069563..2069810
• Replicon: chromosome
• Accession: NZ_CP075344
• Organism: Bacillus subtilis subsp. subtilis strain A1 - Midalam

Toxin (Protein)

• Gene name: bsrE
• Uniprot ID: -
• Locus tag: KIL00_RS10330
• Protein ID: WP_213783630.1
• Coordinates: 2069563..2069655 (+)
• Length: 31 a.a.

Antitoxin (RNA)

Gene name	as-bsrE
Locus tag	-
Coordinates	2069636..2069810 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIL00_RS10315	2064722..2065057	+	336	WP_009967409.1	hypothetical protein	-
KIL00_RS10320	2065104..2068709	-	3606	WP_004399367.1	P-loop NTPase fold protein	-
KIL00_RS10325	2068942..2069301	-	360	WP_213783629.1	hypothetical protein	-
KIL00_RS10330	2069563..2069655	+	93	WP_213783630.1	putative holin-like toxin	Toxin
-	2069636..2069810	-	175	-	-	Antitoxin
KIL00_RS10335	2069925..2070767	-	843	WP_003231327.1	hypothetical protein	-
KIL00_RS10340	2070967..2071425	-	459	WP_041336410.1	type VII secretion system immunity protein YobK	-
KIL00_RS10345	2071435..2073237	-	1803	WP_213783631.1	LXG family T7SS effector ribonuclease toxin YobL	-
KIL00_RS10350	2073339..2073896	-	558	WP_029318053.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2055525..2079227	23702

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-24)

Sequences

Toxin

Protein

• Length: 31 a.a.
• Molecular weight: 3420.23 Da
• Isoelectric Point: 4.1840

>T203468 WP_213783630.1 NZ_CP075344:2069563-2069655 [Bacillus subtilis subsp. subtilis]
MSTFQVLMLMLAFGSFIIALLTYIEKIDLP

Nucleotide

• Length: 93 bp

>T203468 NZ_CP100098:343872-344240 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Nucleotide

• Length: 175 bp

>AT203468 NZ_CP075344:c2069810-2069636 [Bacillus subtilis subsp. subtilis]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTA
TGTACAAAAAGCTGTCCATCAAAGGGCTTGCTCGATGTGGTTTCTAGGTAGACCTATCCCTTCAGGTTCTGAGGCTCAAG
GGAGGTCTATTTTTT

Structures

Antitoxin