Detailed information of TA system TA203452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2861..3512
• Replicon: plasmid p1
• Accession: NZ_CP075324
• Organism: Acinetobacter sp. BHS4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: KIP84_RS18685
• Protein ID: WP_000269904.1
• Coordinates: 2861..3217 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KIP84_RS18690
• Protein ID: WP_001129974.1
• Coordinates: 3210..3512 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIP84_RS18675 (KIP84_18680)	1..924	+	924	WP_000845851.1	replication initiation protein RepM	-
KIP84_RS18680 (KIP84_18685)	992..1537	+	546	WP_000736775.1	plasmid replication DNA-binding protein	-
KIP84_RS18685 (KIP84_18690)	2861..3217	+	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIP84_RS18690 (KIP84_18695)	3210..3512	+	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
KIP84_RS18695 (KIP84_18700)	3505..3714	+	210	WP_000069475.1	hypothetical protein	-
KIP84_RS18700 (KIP84_18705)	3889..4407	+	519	WP_000447195.1	tetratricopeptide repeat protein	-
KIP84_RS18705 (KIP84_18710)	4446..4958	+	513	WP_001089574.1	SMI1/KNR4 family protein	-
KIP84_RS18710 (KIP84_18715)	5065..5382	-	318	WP_000900860.1	hypothetical protein	-
KIP84_RS18715 (KIP84_18720)	5587..5778	-	192	WP_000102616.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..7145	7145

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T203452 WP_000269904.1 NZ_CP075324:2861-3217 [Acinetobacter sp. BHS4]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T203452 NZ_CP100094:343872-344240 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT203452 WP_001129974.1 NZ_CP075324:3210-3512 [Acinetobacter sp. BHS4]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT203452 NZ_CP100094:343654-343875 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: N8YFZ1