Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 4065011..4065608 Replicon chromosome
Accession NZ_CP075316
Organism Klebsiella pneumoniae strain DD521

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KJA60_RS19715 Protein ID WP_004142563.1
Coordinates 4065291..4065608 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KJA60_RS19710 Protein ID WP_004142561.1
Coordinates 4065011..4065298 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJA60_RS19680 (4061091) 4061091..4061339 + 249 WP_020325243.1 DUF1158 domain-containing protein -
KJA60_RS19685 (4061357) 4061357..4061698 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KJA60_RS19690 (4061729) 4061729..4062844 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
KJA60_RS19695 (4063024) 4063024..4063605 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
KJA60_RS19700 (4063605) 4063605..4063973 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
KJA60_RS19705 (4064093) 4064093..4064746 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KJA60_RS19710 (4065011) 4065011..4065298 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KJA60_RS19715 (4065291) 4065291..4065608 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KJA60_RS19720 (4065793) 4065793..4066836 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
KJA60_RS19725 (4067506) 4067506..4068372 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KJA60_RS19730 (4068481) 4068481..4069908 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T203440 WP_004142563.1 NZ_CP075316:c4065608-4065291 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T203440 NZ_CP100087:2612954-2613056 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT203440 WP_004142561.1 NZ_CP075316:c4065298-4065011 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT203440 NZ_CP100087:c2613063-2612918 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References