Detailed information of TA system TA020344

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1315624..1316237
• Replicon: chromosome
• Accession: NC_002678
• Organism: Mesorhizobium loti MAFF303099

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q98K96
• Locus tag: MAFF_RS07420
• Protein ID: WP_010910271.1
• Coordinates: 1315624..1315920 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MAFF_RS07425
• Protein ID: WP_010910272.1
• Coordinates: 1315929..1316237 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAFF_RS07410	1313526..1314824	+	1299	WP_010910269.1	DUF3419 family protein	-
MAFF_RS07415	1314821..1315504	+	684	WP_010910270.1	class I SAM-dependent methyltransferase	-
MAFF_RS07420	1315624..1315920	+	297	WP_010910271.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MAFF_RS07425	1315929..1316237	+	309	WP_010910272.1	HigA family addiction module antidote protein	Antitoxin
MAFF_RS07430	1316313..1317692	-	1380	WP_044548043.1	serine hydrolase	-
MAFF_RS07435	1317981..1318781	+	801	WP_010910274.1	glycoside hydrolase family 25 protein	-
MAFF_RS07440	1319196..1320374	+	1179	WP_010910275.1	lytic murein transglycosylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11641.05 Da
• Isoelectric Point: 6.7266

>T20344 WP_010910271.1 NC_002678:1315624-1315920 [Mesorhizobium japonicum MAFF 303099]
MIVGFRDGWLRAFFVDDTRSRNIPSDLESRLFRKLQMIDDATVDQDLRVPPSNHFEKLRGNLEGFHSIRVNQQWRLIFRW
DGGRGEASDIYLDDHSYK

Nucleotide

• Length: 297 bp

>T20344 NC_002678:1315624-1315920 [Mesorhizobium japonicum MAFF 303099]
ATGATCGTCGGTTTTAGAGACGGATGGCTGCGGGCTTTCTTTGTAGACGACACCCGCTCCCGCAACATTCCGTCCGATCT
CGAAAGCCGCTTGTTCCGCAAGCTCCAGATGATTGACGACGCCACGGTCGATCAGGATTTGCGCGTACCGCCCAGCAACC
ATTTCGAGAAGTTGCGCGGCAATCTCGAAGGCTTCCATTCGATCCGCGTCAACCAGCAGTGGCGACTGATCTTCCGCTGG
GACGGCGGTCGCGGTGAAGCGTCGGACATCTATCTCGACGACCACAGTTATAAGTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11336.07 Da
• Isoelectric Point: 9.4051

>AT20344 WP_010910272.1 NC_002678:1315929-1316237 [Mesorhizobium japonicum MAFF 303099]
MLMTARKPTTVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRSDLW
AVMNSPDERARVDRAKPLATAA

Nucleotide

• Length: 309 bp

>AT20344 NC_002678:1315929-1316237 [Mesorhizobium japonicum MAFF 303099]
ATGTTGATGACCGCACGCAAGCCAACAACGGTTGGCGAGATTCTCACCGAAGAGTTCATGCAGCCGCTCGGCTTGACGCA
GGCGGCTTTGGCCGAGGCGATGGGCGTTCAGCGCAAGCATGTGAACGAATTGTGCAACGACCGCCGCAACGTGACGGCGG
CAACCGCGCTCATTCTGGCCCGGGTGTTTGGCAACAGCCCGGACTTCTGGCTGAACGTCCAACGCCGCAGCGATCTTTGG
GCGGTTATGAATTCGCCCGACGAGCGGGCACGTGTTGACCGCGCCAAGCCTTTGGCGACAGCCGCATAG

Structures

Toxin

• AlphaFold DB: Q98K96