Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324534..325120 Replicon chromosome
Accession NZ_CP075286
Organism Klebsiella pneumoniae strain YTF44-1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KI725_RS01575 Protein ID WP_002920800.1
Coordinates 324752..325120 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag KI725_RS01570 Protein ID WP_002920802.1
Coordinates 324534..324755 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI725_RS01550 (KI725_01550) 320691..321617 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KI725_RS01555 (KI725_01555) 321614..322891 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
KI725_RS01560 (KI725_01560) 322888..323655 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KI725_RS01565 (KI725_01565) 323657..324370 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KI725_RS01570 (KI725_01570) 324534..324755 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KI725_RS01575 (KI725_01575) 324752..325120 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KI725_RS01580 (KI725_01580) 325393..326709 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KI725_RS01585 (KI725_01585) 326816..327703 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KI725_RS01590 (KI725_01590) 327700..328545 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KI725_RS01595 (KI725_01595) 328547..329617 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320691..330354 9663


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T203405 WP_002920800.1 NZ_CP075286:324752-325120 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T203405 NZ_CP100076:634816-635157 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT203405 WP_002920802.1 NZ_CP075286:324534-324755 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT203405 NZ_CP100076:634478-634795 [Klebsiella pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References