Detailed information of TA system TA203349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4462497..4463099
• Replicon: chromosome
• Accession: NZ_CP075174
• Organism: Salmonella enterica subsp. houtenae serovar 45:gz51:- strain 20-369

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A727Z0Z6
• Locus tag: KI721_RS21350
• Protein ID: WP_000576209.1
• Coordinates: 4462788..4463099 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KI721_RS21345
• Protein ID: WP_002954830.1
• Coordinates: 4462497..4462787 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI721_RS21330 (4459973)	4459973..4460875	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
KI721_RS21335 (4460872)	4460872..4461507	+	636	WP_000829029.1	formate dehydrogenase cytochrome b556 subunit	-
KI721_RS21340 (4461504)	4461504..4462433	+	930	WP_079812491.1	formate dehydrogenase accessory protein FdhE	-
KI721_RS21345 (4462497)	4462497..4462787	-	291	WP_002954830.1	NadS family protein	Antitoxin
KI721_RS21350 (4462788)	4462788..4463099	-	312	WP_000576209.1	hypothetical protein	Toxin
KI721_RS21355 (4463329)	4463329..4463815	+	487	Protein_4173	alpha/beta hydrolase	-
KI721_RS21360 (4463819)	4463819..4464760	-	942	WP_024134888.1	fatty acid biosynthesis protein FabY	-
KI721_RS21365 (4464805)	4464805..4465242	-	438	WP_079812492.1	D-aminoacyl-tRNA deacylase	-
KI721_RS21370 (4465239)	4465239..4466111	-	873	WP_000921428.1	virulence factor BrkB family protein	-
KI721_RS21375 (4466105)	4466105..4466704	-	600	WP_079812493.1	glucose-1-phosphatase	-
KI721_RS21380 (4466895)	4466895..4467698	-	804	WP_000059684.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4442444..4463472	21028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12170.12 Da
• Isoelectric Point: 9.8028

>T203349 WP_000576209.1 NZ_CP075174:c4463099-4462788 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
MIFIETEIFTEDVKNLLDDDEYHRLQLFLAIQPDSGDLIQDAGGLRKVRWGVRGRGKRSGVRIIYFYRVSRSEIRLLLIY
KKGIKDDLTAQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T203349 NZ_CP099965:3038266-3038475 [Halomonas sp. DN3]
GTGGTCGCCGAACGGACTGATGGCCAGATTGAGGAAAGGGCAGAACTGCTGATGCTGCAACCGCTACTCGGCGTAGAGCG
CCAAGGTATTCCTGGTCGCTTGCTAATCCTTCCGGAAGTATCGCTTCTGATCTCCTATCAGTTGGCAGGCTCAGATATCC
GGATCATGCGGGTCCTTCACCAAAAGCAGCAATTCCCGCCCGCCGCTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10693.30 Da
• Isoelectric Point: 9.8827

>AT203349 WP_002954830.1 NZ_CP075174:c4462787-4462497 [Salmonella enterica subsp. houtenae serovar 45:g,z51:-]
MDKALFERLTKSMSQMNEIVEGARQPSRTFYIDAMKIKEIRQASGLSQTKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPEHVISALGC

Nucleotide

• Length: 291 bp

>AT203349 NZ_CP099965:3037902-3038231 [Halomonas sp. DN3]
ATGGATACCCGTATACAGTTTCGCATCGATGAAGAGACAAAGCGCCTAGCCCAGCAGATGGCAGAGAGCCAGGGGCGTAC
GCTCAGTGGCGCCTGCAGGGAATTGGCAGAACAACTCGCCGAGCAACAGCGCCAAGCAACGTCACACCATGACTGGTTAT
CGCTTCAGGTCAAGCGAGCGTTTGACGGACTCGAAGCCGGTGCAGCGACCTTTGTCGATCATGAAACCGCAAAATCCCGG
ATGGCAGAACGAAAGTCACGGATCCGCGCCCGTGACCTTGCCCCGGTAATCGGCGATTCCATGGGAAGAAGAGGGTCTGA
CAAGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A727Z0Z6