Detailed information of TA system TA020333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 4083453..4084252
• Replicon: chromosome
• Accession: NC_002655
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: Z_RS21090
• Protein ID: WP_000347273.1
• Coordinates: 4083788..4084252 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: Z_RS21085
• Protein ID: WP_001302819.1
• Coordinates: 4083453..4083788 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z_RS21070 (Z4478)	4079238..4080008	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
Z_RS21075 (Z4479)	4080024..4081358	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
Z_RS21080 (Z4480)	4081733..4083304	+	1572	WP_001273777.1	galactarate dehydratase	-
Z_RS21085 (Z4481)	4083453..4083788	+	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
Z_RS21090 (Z4482)	4083788..4084252	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
Z_RS21095 (Z4483)	4084307..4085116	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
Z_RS21100 (Z4484)	4085365..4086645	+	1281	WP_000681945.1	tagatose-bisphosphate aldolase subunit KbaZ	-
Z_RS21105 (Z4485)	4086668..4087141	+	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
Z_RS21110 (Z4486)	4087152..4087931	+	780	WP_000406223.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
Z_RS21115 (Z4487)	4087921..4088799	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
Z_RS21120 (Z4488)	4088817..4089251	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T20333 WP_000347273.1 NC_002655:4083788-4084252 [Escherichia coli O157:H7 str. EDL933]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T20333 NC_002655:4083788-4084252 [Escherichia coli O157:H7 str. EDL933]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT20333 WP_001302819.1 NC_002655:4083453-4083788 [Escherichia coli O157:H7 str. EDL933]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT20333 NC_002655:4083453-4083788 [Escherichia coli O157:H7 str. EDL933]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7