Detailed information of TA system TA203295

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4659813..4660567
• Replicon: chromosome
• Accession: NZ_CP075141
• Organism: Salmonella enterica strain CFSAN033950

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: ACV97_RS22670
• Protein ID: WP_000558166.1
• Coordinates: 4659813..4660124 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ACV97_RS22675
• Protein ID: WP_001259011.1
• Coordinates: 4660121..4660567 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ACV97_RS22640 (4655471)	4655471..4656373	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
ACV97_RS22645 (4656370)	4656370..4657005	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
ACV97_RS22650 (4657002)	4657002..4657931	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
ACV97_RS22655 (4657978)	4657978..4658268	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
ACV97_RS22660 (4658269)	4658269..4658580	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
ACV97_RS22665 (4658798)	4658798..4659727	+	930	WP_001127703.1	alpha/beta hydrolase	-
ACV97_RS22670 (4659813)	4659813..4660124	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
ACV97_RS22675 (4660121)	4660121..4660567	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
ACV97_RS22680 (4660582)	4660582..4661523	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
ACV97_RS22685 (4661568)	4661568..4662005	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
ACV97_RS22690 (4662002)	4662002..4662874	-	873	WP_000921427.1	virulence factor BrkB family protein	-
ACV97_RS22695 (4662868)	4662868..4663467	-	600	WP_000965695.1	glucose-1-phosphatase	-
ACV97_RS22700 (4663658)	4663658..4664461	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
ACV97_RS22705 (4664495)	4664495..4665391	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T203295 WP_000558166.1 NZ_CP075141:4659813-4660124 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T203295 NZ_CP099907:4095790-4096131 [Escherichia albertii]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCACCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGAGTTTATTGGGGTAATTAC
AGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT203295 WP_001259011.1 NZ_CP075141:4660121-4660567 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT203295 NZ_CP099907:c4095795-4095719 [Escherichia albertii]
AGTCATGATTACTATTCTCCAGAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0R9ME90