Detailed information of TA system TA203209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4680481..4681235
• Replicon: chromosome
• Accession: NZ_CP075135
• Organism: Salmonella enterica strain CFSAN044888

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: A7S10_RS22690
• Protein ID: WP_000558166.1
• Coordinates: 4680481..4680792 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A7S10_RS22695
• Protein ID: WP_001259011.1
• Coordinates: 4680789..4681235 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7S10_RS22660 (4676139)	4676139..4677041	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
A7S10_RS22665 (4677038)	4677038..4677673	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
A7S10_RS22670 (4677670)	4677670..4678599	+	930	WP_070794361.1	formate dehydrogenase accessory protein FdhE	-
A7S10_RS22675 (4678646)	4678646..4678936	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
A7S10_RS22680 (4678937)	4678937..4679248	-	312	WP_001159631.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
A7S10_RS22685 (4679466)	4679466..4680395	+	930	WP_001646963.1	alpha/beta hydrolase	-
A7S10_RS22690 (4680481)	4680481..4680792	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
A7S10_RS22695 (4680789)	4680789..4681235	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
A7S10_RS22700 (4681250)	4681250..4682191	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
A7S10_RS22705 (4682236)	4682236..4682673	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
A7S10_RS22710 (4682670)	4682670..4683542	-	873	WP_000921423.1	virulence factor BrkB family protein	-
A7S10_RS22715 (4683536)	4683536..4684135	-	600	WP_001646959.1	glucose-1-phosphatase	-
A7S10_RS22720 (4684326)	4684326..4685129	-	804	WP_023137059.1	DeoR/GlpR family DNA-binding transcription regulator	-
A7S10_RS22725 (4685163)	4685163..4686059	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T203209 WP_000558166.1 NZ_CP075135:4680481-4680792 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T203209 NZ_CP099894:2758-3045 [Escherichia albertii]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGTGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT203209 WP_001259011.1 NZ_CP075135:4680789-4681235 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT203209 NZ_CP099894:2510-2761 [Escherichia albertii]
ATGCCCAGTATCATTTTGTCTGATACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGTTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A0R9ME90