Detailed information of TA system TA020315

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 283929..284468
• Replicon: chromosome
• Accession: NC_002655
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: Z_RS01320
• Protein ID: WP_001303998.1
• Coordinates: 283929..284189 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: Z_RS01325
• Protein ID: WP_000729705.1
• Coordinates: 284208..284468 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Z_RS01305 (Z0280)	281612..282190	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
Z_RS01310 (Z0281)	282295..283062	+	768	WP_001301698.1	class II glutamine amidotransferase	-
Z_RS01315 (Z0282)	283033..283773	-	741	WP_001225679.1	murein L,D-transpeptidase	-
Z_RS01320 (Z0284)	283929..284189	-	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
Z_RS01325 (Z0285)	284208..284468	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
Z_RS01330 (Z0287)	284654..285427	+	774	WP_000543891.1	C40 family peptidase	-
Z_RS31550	285603..286100	+	498	Protein_249	REP-associated tyrosine transposase RayT	-
Z_RS01340 (Z0290)	286245..287957	-	1713	Protein_250	flagellar biosynthesis protein FlhA	-
Z_RS01345 (Z0291)	287929..288714	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T20315 WP_001303998.1 NC_002655:c284189-283929 [Escherichia coli O157:H7 str. EDL933]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T20315 NC_002655:c284189-283929 [Escherichia coli O157:H7 str. EDL933]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT20315 WP_000729705.1 NC_002655:c284468-284208 [Escherichia coli O157:H7 str. EDL933]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT20315 NC_002655:c284468-284208 [Escherichia coli O157:H7 str. EDL933]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA