Detailed information of TA system TA202904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4606073..4606827
• Replicon: chromosome
• Accession: NZ_CP075110
• Organism: Salmonella enterica strain CFSAN060804

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: CIC27_RS22615
• Protein ID: WP_000558166.1
• Coordinates: 4606073..4606384 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CIC27_RS22620
• Protein ID: WP_001259009.1
• Coordinates: 4606381..4606827 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIC27_RS22585 (4601731)	4601731..4602633	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
CIC27_RS22590 (4602630)	4602630..4603265	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
CIC27_RS22595 (4603262)	4603262..4604191	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
CIC27_RS22600 (4604238)	4604238..4604528	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
CIC27_RS22605 (4604529)	4604529..4604840	-	312	WP_001159631.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
CIC27_RS22610 (4605058)	4605058..4605987	+	930	WP_001127691.1	alpha/beta hydrolase	-
CIC27_RS22615 (4606073)	4606073..4606384	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
CIC27_RS22620 (4606381)	4606381..4606827	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
CIC27_RS22625 (4606842)	4606842..4607783	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
CIC27_RS22630 (4607828)	4607828..4608265	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
CIC27_RS22635 (4608262)	4608262..4609134	-	873	WP_000921423.1	virulence factor BrkB family protein	-
CIC27_RS22640 (4609128)	4609128..4609727	-	600	WP_000965695.1	glucose-1-phosphatase	-
CIC27_RS22645 (4609918)	4609918..4610721	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
CIC27_RS22650 (4610755)	4610755..4611651	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T202904 WP_000558166.1 NZ_CP075110:4606073-4606384 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T202904 NZ_CP099748:4066680-4066898 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT202904 WP_001259009.1 NZ_CP075110:4606381-4606827 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT202904 NZ_CP099748:4066279-4066653 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0R9ME90