Detailed information of TA system TA202861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4476489..4477243
• Replicon: chromosome
• Accession: NZ_CP075108
• Organism: Salmonella enterica strain CFSAN060808

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5U0UUS4
• Locus tag: CIC23_RS21480
• Protein ID: WP_023261623.1
• Coordinates: 4476489..4476800 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CIC23_RS21485
• Protein ID: WP_001259011.1
• Coordinates: 4476797..4477243 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CIC23_RS21450 (4472147)	4472147..4473049	+	903	WP_000331362.1	formate dehydrogenase subunit beta	-
CIC23_RS21455 (4473046)	4473046..4473681	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
CIC23_RS21460 (4473678)	4473678..4474607	+	930	WP_052929816.1	formate dehydrogenase accessory protein FdhE	-
CIC23_RS21465 (4474654)	4474654..4474944	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
CIC23_RS21470 (4474945)	4474945..4475256	-	312	WP_001159632.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
CIC23_RS21475 (4475474)	4475474..4476403	+	930	WP_001127705.1	alpha/beta hydrolase	-
CIC23_RS21480 (4476489)	4476489..4476800	+	312	WP_023261623.1	type II toxin-antitoxin system HigB family toxin	Toxin
CIC23_RS21485 (4476797)	4476797..4477243	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
CIC23_RS21490 (4477258)	4477258..4478199	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
CIC23_RS21495 (4478244)	4478244..4478681	-	438	WP_000560969.1	D-aminoacyl-tRNA deacylase	-
CIC23_RS21500 (4478678)	4478678..4479550	-	873	WP_000921423.1	virulence factor BrkB family protein	-
CIC23_RS21505 (4479544)	4479544..4480143	-	600	WP_000965695.1	glucose-1-phosphatase	-
CIC23_RS21510 (4480334)	4480334..4481137	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
CIC23_RS21515 (4481171)	4481171..4482067	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12418.42 Da
• Isoelectric Point: 9.5921

>T202861 WP_023261623.1 NZ_CP075108:4476489-4476800 [Salmonella enterica]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKIFTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T202861 NZ_CP099721:4123465-4123806 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCACTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT202861 WP_001259011.1 NZ_CP075108:4476797-4477243 [Salmonella enterica]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT202861 NZ_CP099721:c4123470-4123394 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A5U0UUS4