Detailed information of TA system TA020284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1778757..1779298
• Replicon: chromosome
• Accession: NC_002488
• Organism: Xylella fastidiosa 9a5c

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: XF_RS08055
• Protein ID: WP_031336866.1
• Coordinates: 1778757..1779080 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q9PCB8
• Locus tag: XF_RS08060
• Protein ID: WP_010894329.1
• Coordinates: 1779077..1779298 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XF_RS08035 (XF1858)	1775701..1776393	+	693	WP_031336589.1	7-cyano-7-deazaguanine synthase QueC	-
XF_RS13385	1776528..1776899	-	372	Protein_1575	hypothetical protein	-
XF_RS08045 (XF1859)	1777003..1778181	+	1179	WP_010894325.1	replication initiation factor domain-containing protein	-
XF_RS08050 (XF1860)	1778168..1778500	+	333	WP_010894326.1	hypothetical protein	-
XF_RS14100 (XF1861)	1778513..1778668	+	156	WP_155114993.1	hypothetical protein	-
XF_RS08055 (XF1862)	1778757..1779080	-	324	WP_031336866.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XF_RS08060 (XF1863)	1779077..1779298	-	222	WP_010894329.1	antitoxin MazE family protein	Antitoxin
XF_RS08065 (XF1864)	1779582..1780016	-	435	WP_010894330.1	DUF3693 domain-containing protein	-
XF_RS08070 (XF1865)	1780157..1780459	+	303	WP_010894331.1	hypothetical protein	-
XF_RS14105	1780446..1780760	+	315	WP_143704178.1	hypothetical protein	-
XF_RS08075 (XF1866)	1780764..1780946	+	183	WP_023906503.1	hypothetical protein	-
XF_RS13395 (XF1867)	1781514..1781942	+	429	Protein_1585	hypothetical protein	-
XF_RS08085 (XF1868)	1781946..1782613	+	668	Protein_1586	hypothetical protein	-
XF_RS08090 (XF1869)	1782606..1782890	+	285	WP_010894335.1	DUF2523 domain-containing protein	-
XF_RS08095 (XF1870)	1782890..1783960	+	1071	WP_010894336.1	zonular occludens toxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP009790 Xylella fastidiosa strain U24d Prophage TA049820 1775287..1791435 0	inside	Prophage	-	-	1775302..1791449	16147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11771.81 Da
• Isoelectric Point: 7.9848

>T20284 WP_031336866.1 NC_002488:c1779080-1778757 [Xylella fastidiosa 9a5c]
MIQRGDLVTVSLQGDYGKPRPALIVQSDLLTELDSVALCPVTSDLRNAIFRVTVEPTAANGLRTLSQVMVDKISTLPRNK
ISEPFGRLNDERMKAIERALLLIIGII

Nucleotide

• Length: 324 bp

>T20284 NC_002488:c1779080-1778757 [Xylella fastidiosa 9a5c]
ATGATTCAACGTGGCGACCTTGTAACAGTTTCCCTACAAGGCGACTACGGCAAGCCTAGACCAGCGTTGATCGTTCAATC
AGACCTTCTAACGGAGTTAGATAGTGTCGCACTGTGTCCGGTCACGAGCGACCTGCGGAATGCGATATTTCGTGTCACCG
TTGAACCAACTGCGGCCAATGGTTTACGGACACTGTCGCAGGTCATGGTAGATAAAATTTCAACACTACCACGTAACAAA
ATCAGCGAACCTTTTGGACGTCTCAATGACGAGAGAATGAAAGCAATAGAAAGAGCATTGTTACTAATTATTGGTATCAT
TTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8426.53 Da
• Isoelectric Point: 7.3606

>AT20284 WP_010894329.1 NC_002488:c1779298-1779077 [Xylella fastidiosa 9a5c]
MTSTHRPDSKIVHHREQMRAAGLRPVQLWVPDTRTPEFAAEIQSQCRALKGDQAEADALRFTEKAMTHIEDWK

Nucleotide

• Length: 222 bp

>AT20284 NC_002488:c1779298-1779077 [Xylella fastidiosa 9a5c]
ATGACATCAACTCACAGACCAGACAGCAAGATAGTTCACCACAGGGAACAAATGCGCGCAGCTGGATTGCGTCCTGTGCA
GCTCTGGGTACCGGATACGCGCACACCGGAATTCGCTGCTGAAATTCAAAGCCAGTGCCGCGCCCTGAAAGGCGACCAGG
CAGAAGCCGACGCTTTACGCTTCACAGAAAAAGCCATGACCCATATTGAAGATTGGAAATGA

Structures

Antitoxin

• AlphaFold DB: Q9PCB8