Detailed information of TA system TA202798

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 100170..100423
• Replicon: plasmid pCTX_J9_3
• Accession: NZ_CP075064
• Organism: Escherichia coli strain EC_J_9

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H4F96_RS25185
• Protein ID: WP_001312851.1
• Coordinates: 100274..100423 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	100170..100229 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F96_RS25160 (96897)	96897..97643	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
H4F96_RS25165 (97698)	97698..98258	+	561	WP_000139328.1	fertility inhibition protein FinO	-
H4F96_RS25170 (98390)	98390..98590	+	201	WP_015059022.1	hypothetical protein	-
H4F96_RS25175 (98976)	98976..99575	+	600	WP_032083981.1	PIN domain-containing protein	-
H4F96_RS25180 (99652)	99652..99969	+	318	WP_153449145.1	hypothetical protein	-
- (100170)	100170..100229	-	60	NuclAT_1	-	Antitoxin
- (100170)	100170..100229	-	60	NuclAT_1	-	Antitoxin
- (100170)	100170..100229	-	60	NuclAT_1	-	Antitoxin
- (100170)	100170..100229	-	60	NuclAT_1	-	Antitoxin
H4F96_RS25185 (100274)	100274..100423	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
H4F96_RS25190 (100707)	100707..100955	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..101266	101266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T202798 WP_001312851.1 NZ_CP075064:100274-100423 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T202798 NZ_CP099623:3941305-3941586 [Paraburkholderia fungorum]
ATGATCACGACATTCGGCGACAAGGCTACGGCAGCAATCTTCCACGGCAAGTTCGTGCATTCACTGCCGCTCTACATGCA
GGCGCTCGCACGCCGCAAGCTGCTGATGATCGATGCCGCGGAGTCCATCCGCAGCCTGCATGCGCCGCCGGGCAACCGGC
TCGAAGCCTTGCAAGGCCAGCGCAGCGGGCAGTGGAGCATTCGCATCAACGCGCAGTGGCGCATCTGTTTTCATTTTGTC
GACGGGGAGGCGCTGAATGTCGAAATTGTCGACTATCACTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT202798 NZ_CP075064:c100229-100170 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin