Detailed information of TA system TA202795

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60692..60961
• Replicon: plasmid pCTX_J9_3
• Accession: NZ_CP075064
• Organism: Escherichia coli strain EC_J_9

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: H4F96_RS24955
• Protein ID: WP_001372321.1
• Coordinates: 60836..60961 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	60692..60757 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F96_RS24925	56402..56929	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H4F96_RS24930	56987..57220	+	234	WP_000006003.1	DUF905 family protein	-
H4F96_RS24935	57281..59304	+	2024	Protein_74	ParB/RepB/Spo0J family partition protein	-
H4F96_RS24940	59373..59807	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H4F96_RS24945	59804..60566	+	763	Protein_76	plasmid SOS inhibition protein A	-
-	60535..60759	+	225	NuclAT_0	-	-
-	60535..60759	+	225	NuclAT_0	-	-
-	60535..60759	+	225	NuclAT_0	-	-
-	60535..60759	+	225	NuclAT_0	-	-
H4F96_RS24950	60544..60723	-	180	WP_001309233.1	hypothetical protein	-
-	60692..60757	-	66	-	-	Antitoxin
H4F96_RS25895	60745..60894	+	150	Protein_78	plasmid maintenance protein Mok	-
H4F96_RS24955	60836..60961	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
H4F96_RS24960	61280..61576	-	297	Protein_80	hypothetical protein	-
H4F96_RS24965	61876..62172	+	297	WP_001272251.1	hypothetical protein	-
H4F96_RS24970	62283..63104	+	822	WP_001234445.1	DUF932 domain-containing protein	-
H4F96_RS24975	63401..64048	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
H4F96_RS24980	64325..64708	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
H4F96_RS24985	64899..65585	+	687	WP_015059009.1	PAS domain-containing protein	-
H4F96_RS24990	65679..65906	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..101266	101266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T202795 WP_001372321.1 NZ_CP075064:60836-60961 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T202795 NZ_CP099610:4810489-4810743 [Pseudomonas moraviensis]
ATGAAAATCGAGTTCACGCCCACAGGCTGGGAAGACTATTTGTGGTTTCAGCAAAACGACAAGGCTGGGCTCAAGCGCAT
CAATCTTCTGATCAAAGCAATCAAGCGCGAACCGTTTGAGGGTGTCGGTAAACCAGAGGCGCTCAAACATAATATGAGCG
GCCTCTGGTCACGCCGAGTAACGGCCGAACATCGTCTGGTGTACAGGGTTGAGGACGGCGAAATCTGCGTCATCAGTTGC
CGGTATCACTATTGA

Antitoxin

Nucleotide

• Length: 66 bp

>AT202795 NZ_CP075064:c60757-60692 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin