Detailed information of TA system TA202786

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 76658..76912
• Replicon: plasmid pOQX_J9_2
• Accession: NZ_CP075063
• Organism: Escherichia coli strain EC_J_9

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H4F96_RS24195
• Protein ID: WP_001312851.1
• Coordinates: 76658..76807 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	76851..76912 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F96_RS24155 (72210)	72210..72611	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
H4F96_RS24160 (72544)	72544..72801	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
H4F96_RS24165 (72894)	72894..73547	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
H4F96_RS24170 (73645)	73645..73785	-	141	WP_001333237.1	hypothetical protein	-
H4F96_RS24175 (74486)	74486..75343	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
H4F96_RS24180 (75336)	75336..75818	-	483	WP_181726436.1	hypothetical protein	-
H4F96_RS25855 (75811)	75811..75858	-	48	WP_229471593.1	hypothetical protein	-
H4F96_RS25860 (75849)	75849..76100	+	252	WP_223195197.1	replication protein RepA	-
H4F96_RS24190 (76117)	76117..76374	-	258	WP_000083845.1	replication regulatory protein RepA	-
H4F96_RS24195 (76658)	76658..76807	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (76851)	76851..76912	+	62	NuclAT_1	-	Antitoxin
- (76851)	76851..76912	+	62	NuclAT_1	-	Antitoxin
- (76851)	76851..76912	+	62	NuclAT_1	-	Antitoxin
- (76851)	76851..76912	+	62	NuclAT_1	-	Antitoxin
H4F96_RS24200 (77168)	77168..77242	-	75	Protein_81	endonuclease	-
H4F96_RS24205 (77488)	77488..77700	-	213	WP_001299730.1	ANR family transcriptional regulator	-
H4F96_RS24210 (77836)	77836..78396	-	561	WP_000139315.1	fertility inhibition protein FinO	-
H4F96_RS24215 (78499)	78499..79359	-	861	WP_000704514.1	alpha/beta hydrolase	-
H4F96_RS24220 (79418)	79418..80164	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..140375	140375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T202786 WP_001312851.1 NZ_CP075063:c76807-76658 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T202786 NZ_CP099610:182932-183213 [Pseudomonas moraviensis]
ATGACTTATAAGCTTGAGTTTCTGCCGTCTGCGCACAAAGAGTGGAACAAGCTGGGTCATACGTTGCGGGAGCAGTTCAA
AAAGAAGCTGGGTGAGAGGCTGAAGCTTCCCAGAATCCCGGCTGACGCCCTCCACAGCATGCCCGACTGCTACAAAATCA
AACTCAAAGCCTCGGGATATCGATTGGTTTACCAAGTCATCGACGAACGAGTCGTGGTTTCCGTGCTAGCGGTTGGCAAG
CGCGAACGCAGCAGCGTTTACGATGCTGCCAAGAACCGTTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT202786 NZ_CP075063:76851-76912 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin