Detailed information of TA system TA202785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4728867..4729469
• Replicon: chromosome
• Accession: NZ_CP075062
• Organism: Escherichia coli strain EC_J_9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H4F96_RS22750
• Protein ID: WP_000897305.1
• Coordinates: 4729158..4729469 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H4F96_RS22745
• Protein ID: WP_000356397.1
• Coordinates: 4728867..4729157 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F96_RS22720 (4724793)	4724793..4725695	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H4F96_RS22725 (4725692)	4725692..4726327	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H4F96_RS22730 (4726324)	4726324..4727253	+	930	WP_001749031.1	formate dehydrogenase accessory protein FdhE	-
H4F96_RS22735 (4727583)	4727583..4727825	-	243	WP_001086388.1	protein YiiF	-
H4F96_RS22740 (4728044)	4728044..4728262	-	219	WP_001295676.1	CopG family transcriptional regulator	-
H4F96_RS22745 (4728867)	4728867..4729157	-	291	WP_000356397.1	NadS family protein	Antitoxin
H4F96_RS22750 (4729158)	4729158..4729469	-	312	WP_000897305.1	hypothetical protein	Toxin
H4F96_RS22755 (4729698)	4729698..4730606	+	909	WP_001162704.1	alpha/beta hydrolase	-
H4F96_RS22760 (4730670)	4730670..4731611	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
H4F96_RS22765 (4731656)	4731656..4732093	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
H4F96_RS22770 (4732090)	4732090..4732962	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H4F96_RS22775 (4732956)	4732956..4733555	-	600	WP_001295269.1	glucose-1-phosphatase	-
H4F96_RS22780 (4733654)	4733654..4734439	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T202785 WP_000897305.1 NZ_CP075062:c4729469-4729158 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T202785 NZ_CP099609:5146002-5146280 [Pseudomonas moraviensis]
ATGATCAAATCCTTTCAGCACAAAGGCCTTCGTGGCTTCTATGAAACAGGTACGACTCGTGGAATTCGTGCCGAGCATGC
AAAACGGCTGTCGCGCATGTTGCAGTTCATGGATCGAGCGACGCTTCCCGGGGACTTGGACTTGCCCGGCTGGCGCCTGC
ATCCTTTGAAAGGTGAGCTAAGCCAATATTGGTCGCTCAGCGTTTCAGGCGACTGGCGAGTGATCTTTCGTTTTGTCGGT
TCGGATATCGAATTGGTCGATTATCTGGACTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT202785 WP_000356397.1 NZ_CP075062:c4729157-4728867 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT202785 NZ_CP099609:5146295-5146582 [Pseudomonas moraviensis]
ATGCCCATGCACAACCCGCCACACCCCGGTGAAACCCTGCTGCTGGATGTCTTGCCCGAGCTTGGCATCAGCGTGAGCGA
ATTGGCCCGGCACCTTGGCTTTGCACGCCCGCACCTTTCGCGCGTACTGCACGGACACGCGCCAATCAGCCCGGATCTGG
CAGTACGACTTGAGCGTGCAGGGATCGGCAAGGCGCGCGTTTGGCTAGGCGTCCAAACCGACTACGATCTTTGGCAAGCG
GAGCACCGTGAGCAACCAATGATAGAACCCCTCGCAGCCCACGGCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7