Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3891036..3891577 Replicon chromosome
Accession NZ_CP075062
Organism Escherichia coli strain EC_J_9

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag H4F96_RS18915 Protein ID WP_000615982.1
Coordinates 3891299..3891577 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H4F96_RS18910 Protein ID WP_000729704.1
Coordinates 3891036..3891296 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F96_RS18885 (3886819) 3886819..3887604 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
H4F96_RS18890 (3887576) 3887576..3889288 + 1713 Protein_3715 flagellar biosynthesis protein FlhA -
H4F96_RS18895 (3889393) 3889393..3889671 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H4F96_RS18900 (3889664) 3889664..3890020 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
H4F96_RS18905 (3890077) 3890077..3890850 - 774 WP_000543899.1 C40 family peptidase -
H4F96_RS18910 (3891036) 3891036..3891296 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H4F96_RS18915 (3891299) 3891299..3891577 + 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H4F96_RS18920 (3891733) 3891733..3892473 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H4F96_RS18925 (3892444) 3892444..3893211 - 768 WP_000333380.1 class II glutamine amidotransferase -
H4F96_RS18930 (3893417) 3893417..3893995 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T202783 WP_000615982.1 NZ_CP075062:3891299-3891577 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T202783 NZ_CP099609:4869037-4869291 [Pseudomonas moraviensis]
ATGCAAACCATCAACTACACCACCGCCCGTGCGCATTTGGCCGAGACGATGGACCGTGTCAATGAAGATCGTGCGCCGCT
ATTGGTCACTCGGCAGAAAGGTGAACCAGTAGTGATGATGTCTTTGGCCGAATACAACGCATTGGAAGAAACAGCGTACT
TGCTTCGCTCCCCCGCCAACGCCGAACGGCTGATGAAATCCATCGGTGAGTTACGCGCCGGCAAAGCCAAAGTCAGGCAA
TTGATCGAAGAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT202783 WP_000729704.1 NZ_CP075062:3891036-3891296 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT202783 NZ_CP099609:4869288-4869548 [Pseudomonas moraviensis]
ATGAAAATCGAGTTCACGCCCACAGGCTGGGAAGACTATTTGTGGTTTCAGCAAAACGACAAGGCTGGGCTCAAGCGCAT
CAATCTTCTGATCAAAGCAATCAAGCGCGAACCGTTTGAGGGTGTCGGTAAACCAGAGGCGCTCAAGCATAATATGAGCG
GCCTCTGGTCACGCCGAGTAACGGCCGAACATCGTCTGGTGTACAGGGTTGAGGACGGCGAAATCTGCGTCATCAGTTGC
CGGTATCACTATTCAACCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References