Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 587902..588701 Replicon chromosome
Accession NZ_CP075062
Organism Escherichia coli strain EC_J_9

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag H4F96_RS02860 Protein ID WP_000347267.1
Coordinates 587902..588366 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H4F96_RS02865 Protein ID WP_001307405.1
Coordinates 588366..588701 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F96_RS02830 (582903) 582903..583337 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
H4F96_RS02835 (583355) 583355..584233 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H4F96_RS02840 (584223) 584223..585002 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H4F96_RS02845 (585013) 585013..585486 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H4F96_RS02850 (585509) 585509..586789 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
H4F96_RS02855 (587038) 587038..587847 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H4F96_RS02860 (587902) 587902..588366 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H4F96_RS02865 (588366) 588366..588701 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H4F96_RS02870 (588850) 588850..590421 - 1572 WP_213190783.1 galactarate dehydratase -
H4F96_RS02875 (590796) 590796..592130 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H4F96_RS02880 (592146) 592146..592916 + 771 WP_032310059.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 587902..599576 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T202763 WP_000347267.1 NZ_CP075062:c588366-587902 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T202763 NZ_CP099602:c2251368-2251099 [Pseudomonas siliginis]
ATGCAGATTGAGTGGCGGCCCGAAGCCAAGGCCGATCTTTCGAACATCCTCAGATACATTGGCGAGAGAAACTTTGCAGC
CGCAGTGAGTCTCTCGAAATCCATCGAAGAATCCACAACCTCACTCTCCGTTCATCCCCATCTCTACCGTAAAGGTCGAT
CTCCCGGCACCCGGGAAATCGTTGTCCATCCTAACTACGTAGTGATTTATGAGGTGACGGATCGAATCGAGATACTTAGC
GTTTTGCATACCCGACAGGAATATCCATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT202763 WP_001307405.1 NZ_CP075062:c588701-588366 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT202763 NZ_CP099602:c2251549-2251358 [Pseudomonas siliginis]
ATGGACGGCAAAATCTTTCCCGACGATTCAGGGTTTGAAAACAACGAGCAGGCTGCCAGTCATGATCGCTGGTTGCGAGC
CAAGGTGCAGGCGTCCAGAGATGATCCCCATCCGAGTCTCCCCCATGAGGACGTGATGGCGGACATGCATGCCTTGATCG
AATCCATGCGAAAAAAGGCCGATGCAGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References