Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4136600..4137399 Replicon chromosome
Accession NZ_CP075061
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag H4F95_RS22980 Protein ID WP_000347267.1
Coordinates 4136935..4137399 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag H4F95_RS22975 Protein ID WP_001307405.1
Coordinates 4136600..4136935 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS22960 (4132385) 4132385..4133155 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
H4F95_RS22965 (4133171) 4133171..4134505 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
H4F95_RS22970 (4134880) 4134880..4136451 + 1572 WP_213206460.1 galactarate dehydratase -
H4F95_RS22975 (4136600) 4136600..4136935 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
H4F95_RS22980 (4136935) 4136935..4137399 + 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
H4F95_RS22985 (4137454) 4137454..4138263 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
H4F95_RS22990 (4138512) 4138512..4139792 + 1281 WP_000681933.1 tagatose-bisphosphate aldolase subunit KbaZ -
H4F95_RS22995 (4139815) 4139815..4140288 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
H4F95_RS23000 (4140299) 4140299..4141078 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
H4F95_RS23005 (4141068) 4141068..4141946 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
H4F95_RS23010 (4141964) 4141964..4142398 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4125944..4137399 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T202760 WP_000347267.1 NZ_CP075061:4136935-4137399 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T202760 NZ_CP099602:475263-475625 [Pseudomonas siliginis]
ATGGTGCGCCGACAGCCCCCTCAGCGGGGTGACGTTTACTGGATTGATCCAAACCCAGTGGCGGGGCGGGAGATGATGAA
CAGACATCGCTTTGTGGTGATCACGCCCAGAGAGATCAACGCATTGGGCGTGAGCATGACGGTGCCTATTACTAGCGGTG
GGAATTTCAGCCGGTATATGGGTTTGGCGGTCGCCATTACTGGCCACGAAACCAATGGTGTAGCGGTGTGTAATCAGGTT
CGCAGTTTTGACATAGAACAACGGGTACGAGACGGCACGGCCAAATTCATCGAGCGACTGGATGACGTGACAATGTCTGA
CATCGTCGACCGTGTAATCAGTGCAATCGACCCGCTAAGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT202760 WP_001307405.1 NZ_CP075061:4136600-4136935 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT202760 NZ_CP099602:474994-475263 [Pseudomonas siliginis]
ATGTCCGTAACTTCCAAGATACGCCGCCAAGGCGGCGCTGCCGTCATTACGATTCCGCCCACGCTTTTAAAGTTGTTGCA
TCTGGAGGTTGGCGCACAGCTAGAGCTGAATGTGGTCGATGGCGAACTGATTGCTCGTCCAGCCGTAGCCGCTACGCGTA
AGCGTTATAGTTTGTCAGAGCTACTCGAAGGCGCAGAAGAATTGCAGAAACTTAACGCCCAGACTAGCGAGACCCTTGAA
GGTGATTCTGTCGGCCGGGAGCTGGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References