Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | prlF-yhaV (relBE)/YhaV-PrlF |
| Location | 4136600..4137399 | Replicon | chromosome |
| Accession | NZ_CP075061 | ||
| Organism | Escherichia coli strain TCM_B_2 | ||
Toxin (Protein)
| Gene name | yhaV | Uniprot ID | U9XVR9 |
| Locus tag | H4F95_RS22980 | Protein ID | WP_000347267.1 |
| Coordinates | 4136935..4137399 (+) | Length | 155 a.a. |
Antitoxin (Protein)
| Gene name | prlF | Uniprot ID | S1EB98 |
| Locus tag | H4F95_RS22975 | Protein ID | WP_001307405.1 |
| Coordinates | 4136600..4136935 (+) | Length | 112 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| H4F95_RS22960 (4132385) | 4132385..4133155 | - | 771 | WP_001058227.1 | 2-dehydro-3-deoxyglucarate aldolase | - |
| H4F95_RS22965 (4133171) | 4133171..4134505 | - | 1335 | WP_000599636.1 | galactarate/glucarate/glycerate transporter GarP | - |
| H4F95_RS22970 (4134880) | 4134880..4136451 | + | 1572 | WP_213206460.1 | galactarate dehydratase | - |
| H4F95_RS22975 (4136600) | 4136600..4136935 | + | 336 | WP_001307405.1 | type II toxin-antitoxin system antitoxin PrlF | Antitoxin |
| H4F95_RS22980 (4136935) | 4136935..4137399 | + | 465 | WP_000347267.1 | type II toxin-antitoxin system ribonuclease toxin YhaV | Toxin |
| H4F95_RS22985 (4137454) | 4137454..4138263 | - | 810 | WP_000072187.1 | aga operon transcriptional regulator AgaR | - |
| H4F95_RS22990 (4138512) | 4138512..4139792 | + | 1281 | WP_000681933.1 | tagatose-bisphosphate aldolase subunit KbaZ | - |
| H4F95_RS22995 (4139815) | 4139815..4140288 | + | 474 | WP_001295547.1 | PTS N-acetylgalactosamine transporter subunit IIB | - |
| H4F95_RS23000 (4140299) | 4140299..4141078 | + | 780 | WP_000406214.1 | PTS N-acetylgalactosamine transporter subunit IIC | - |
| H4F95_RS23005 (4141068) | 4141068..4141946 | + | 879 | WP_001295548.1 | PTS N-acetylgalactosamine transporter subunit IID | - |
| H4F95_RS23010 (4141964) | 4141964..4142398 | + | 435 | WP_000948824.1 | PTS galactosamine/N-acetylgalactosamine transporter subunit IIA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 4125944..4137399 | 11455 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 155 a.a. Molecular weight: 17807.17 Da Isoelectric Point: 9.4947
>T202760 WP_000347267.1 NZ_CP075061:4136935-4137399 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH
Download Length: 465 bp
>T202760 NZ_CP099602:475263-475625 [Pseudomonas siliginis]
ATGGTGCGCCGACAGCCCCCTCAGCGGGGTGACGTTTACTGGATTGATCCAAACCCAGTGGCGGGGCGGGAGATGATGAA
CAGACATCGCTTTGTGGTGATCACGCCCAGAGAGATCAACGCATTGGGCGTGAGCATGACGGTGCCTATTACTAGCGGTG
GGAATTTCAGCCGGTATATGGGTTTGGCGGTCGCCATTACTGGCCACGAAACCAATGGTGTAGCGGTGTGTAATCAGGTT
CGCAGTTTTGACATAGAACAACGGGTACGAGACGGCACGGCCAAATTCATCGAGCGACTGGATGACGTGACAATGTCTGA
CATCGTCGACCGTGTAATCAGTGCAATCGACCCGCTAAGCTGA
ATGGTGCGCCGACAGCCCCCTCAGCGGGGTGACGTTTACTGGATTGATCCAAACCCAGTGGCGGGGCGGGAGATGATGAA
CAGACATCGCTTTGTGGTGATCACGCCCAGAGAGATCAACGCATTGGGCGTGAGCATGACGGTGCCTATTACTAGCGGTG
GGAATTTCAGCCGGTATATGGGTTTGGCGGTCGCCATTACTGGCCACGAAACCAATGGTGTAGCGGTGTGTAATCAGGTT
CGCAGTTTTGACATAGAACAACGGGTACGAGACGGCACGGCCAAATTCATCGAGCGACTGGATGACGTGACAATGTCTGA
CATCGTCGACCGTGTAATCAGTGCAATCGACCCGCTAAGCTGA
Antitoxin
Download Length: 112 a.a. Molecular weight: 12358.94 Da Isoelectric Point: 4.8616
>AT202760 WP_001307405.1 NZ_CP075061:4136600-4136935 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE
Download Length: 336 bp
>AT202760 NZ_CP099602:474994-475263 [Pseudomonas siliginis]
ATGTCCGTAACTTCCAAGATACGCCGCCAAGGCGGCGCTGCCGTCATTACGATTCCGCCCACGCTTTTAAAGTTGTTGCA
TCTGGAGGTTGGCGCACAGCTAGAGCTGAATGTGGTCGATGGCGAACTGATTGCTCGTCCAGCCGTAGCCGCTACGCGTA
AGCGTTATAGTTTGTCAGAGCTACTCGAAGGCGCAGAAGAATTGCAGAAACTTAACGCCCAGACTAGCGAGACCCTTGAA
GGTGATTCTGTCGGCCGGGAGCTGGGTTGA
ATGTCCGTAACTTCCAAGATACGCCGCCAAGGCGGCGCTGCCGTCATTACGATTCCGCCCACGCTTTTAAAGTTGTTGCA
TCTGGAGGTTGGCGCACAGCTAGAGCTGAATGTGGTCGATGGCGAACTGATTGCTCGTCCAGCCGTAGCCGCTACGCGTA
AGCGTTATAGTTTGTCAGAGCTACTCGAAGGCGCAGAAGAATTGCAGAAACTTAACGCCCAGACTAGCGAGACCCTTGAA
GGTGATTCTGTCGGCCGGGAGCTGGGTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XTR4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E0XVC7 |