Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 990542..991083 Replicon chromosome
Accession NZ_CP075061
Organism Escherichia coli strain TCM_B_2

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag H4F95_RS07785 Protein ID WP_000615976.1
Coordinates 990542..990820 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag H4F95_RS07790 Protein ID WP_000729704.1
Coordinates 990823..991083 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H4F95_RS07770 (988124) 988124..988702 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
H4F95_RS07775 (988908) 988908..989675 + 768 WP_000333380.1 class II glutamine amidotransferase -
H4F95_RS07780 (989646) 989646..990386 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
H4F95_RS07785 (990542) 990542..990820 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
H4F95_RS07790 (990823) 990823..991083 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
H4F95_RS07795 (991269) 991269..992042 + 774 WP_000543895.1 C40 family peptidase -
H4F95_RS07800 (992099) 992099..992455 - 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
H4F95_RS07805 (992448) 992448..992726 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
H4F95_RS07810 (992831) 992831..994543 - 1713 Protein_899 flagellar biosynthesis protein FlhA -
H4F95_RS07815 (994515) 994515..995300 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 981593..992726 11133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T202745 WP_000615976.1 NZ_CP075061:c990820-990542 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T202745 NZ_CP099600:c903291-903040 [Pseudomonas siliginis]
ATGTCAAAGACTGAAAAACTTCTCGCCAAACTAGTCAACGGCCAAAGCGCATTCACATGGTCCGAGCTTGTCACTCTGTT
GGCGCGTCTTGGTTACTCTCAGCTGGAAGGATCCGGTAGCAGGATCAAATTCGACAACGGGAATCCGAGCGCAATGATCA
ACCTGCACAAGCCACATCCCGGAAATGAAATCAAAACATACGCAAGACGCCAGATTATCGAGCAACTGAAAACAGGAGGC
CTGATTCCATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT202745 WP_000729704.1 NZ_CP075061:c991083-990823 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT202745 NZ_CP099600:c903043-902702 [Pseudomonas siliginis]
ATGAGTTCCCAGCTGCACTACAAAGGCTATGTTGGCTCTATCGAGGCGAGCACGGAAGATAAGTGCCTGTTCGGAAAACT
CCTTTTCATCAGGGCGCTGGTTAATTATGAAGGACAAACGGTGGCTGAACTGGAAGCAGCCTTTCGCGAGGCGGTAGATG
ACTATTTACAAACCTGTCAGCAGCTCGGGCAGAAGCCTGAAATCCCATGCAAAGGATCGTTCAATGTTCGCATAGGTCAC
GATCTGCATTTAGCAGCGGCTCTGGCGGCAACCCGTCAAAATGTGTCGTTGAATGATCTGACACGTCAAGCACTGAACGA
ATACCTCCAACACCACGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References