Detailed information of TA system TA202742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 136155..136757
• Replicon: chromosome
• Accession: NZ_CP075061
• Organism: Escherichia coli strain TCM_B_2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H4F95_RS03810
• Protein ID: WP_000897305.1
• Coordinates: 136155..136466 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H4F95_RS03815
• Protein ID: WP_000356397.1
• Coordinates: 136467..136757 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F95_RS03780 (131185)	131185..131970	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
H4F95_RS03785 (132069)	132069..132668	+	600	WP_001295269.1	glucose-1-phosphatase	-
H4F95_RS03790 (132662)	132662..133534	+	873	WP_000920762.1	virulence factor BrkB family protein	-
H4F95_RS03795 (133531)	133531..133968	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
H4F95_RS03800 (134013)	134013..134954	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H4F95_RS03805 (135018)	135018..135926	-	909	WP_001162704.1	alpha/beta hydrolase	-
H4F95_RS03810 (136155)	136155..136466	+	312	WP_000897305.1	hypothetical protein	Toxin
H4F95_RS03815 (136467)	136467..136757	+	291	WP_000356397.1	NadS family protein	Antitoxin
H4F95_RS03820 (137362)	137362..137580	+	219	WP_001297075.1	CopG family transcriptional regulator	-
H4F95_RS03825 (137799)	137799..138041	+	243	WP_001086388.1	protein YiiF	-
H4F95_RS03830 (138371)	138371..139300	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H4F95_RS03835 (139297)	139297..139932	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H4F95_RS03840 (139929)	139929..140831	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T202742 WP_000897305.1 NZ_CP075061:136155-136466 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T202742 NZ_CP099599:c4840829-4840485 [Pseudomonas siliginis]
ATGCGAACAATCTTTTTTGAAACAACGATATTTACCAAAAGCGTTGGCCGCTATCTGACGGACGACGAATATCGAACATT
GCAGAACTACCTTCAGAGCAATCCGCTTGCGGGCGATGTAATGCCTCGAACGGGTGGCTTCCGTAAGCTGCGCTGGGCTG
ATTCTCGCCGTGGCAAGGGAAGACGAGGCGGCTTGCGGGTTCTCTACTATTGGCTTATGAATGATGGTCAGTTCTGGATG
TTCGCCATTTACGACAAAGATGAGCTTGAGAAGCTGACTGCCGAGCAAGAGAGGGCACTCAGGACAGCCATTGATAAAGA
GTTGAAAAAACGAGGTACCCCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT202742 WP_000356397.1 NZ_CP075061:136467-136757 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT202742 NZ_CP099599:c4840488-4840174 [Pseudomonas siliginis]
ATGAAAAAACGCGACCTGTTTGCAGAGTTAATGCAGGGCGTTGATGAGATGGCGGCTCAACGCGAGGGCAAGATCACACT
GCGCAATACAGTGCTTGAGGACAAGCCTGTACCTGAAGTGGGCGCACAGGAAATTGTGGCTCTTCGAGAAAAACTGCATA
TGTCTCAAGCTGTTTTTGCCAGTCGAATCAGGACAAGTCCAAACACCCTTCGAAATTGGGAGCAGGAAAAGTCGAAGCCC
AATGCTCAAGCAGCCTTATTGATACGACTGGTTGAAAAATTTCCAGATATGGTTGAGCGCTTGAACACTGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7