Detailed information of TA system TA202738

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 43227..43491
• Replicon: plasmid pCMY_B2_5
• Accession: NZ_CP075060
• Organism: Escherichia coli strain TCM_B_2

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: H4F95_RS03115
• Protein ID: WP_001387489.1
• Coordinates: 43227..43379 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	43431..43491 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H4F95_RS03075 (38273)	38273..38590	+	318	WP_000118520.1	quaternary ammonium compound efflux SMR transporter SugE	-
H4F95_RS03080 (38587)	38587..39120	-	534	WP_001221666.1	lipocalin family protein	-
H4F95_RS03085 (39214)	39214..40359	-	1146	WP_039023136.1	extended-spectrum class C beta-lactamase CMY-42	-
H4F95_RS26325 (41296)	41296..41394	+	99	Protein_48	ethanolamine utilization protein EutE	-
H4F95_RS03095 (41466)	41466..41675	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H4F95_RS03100 (42067)	42067..42243	+	177	WP_001054897.1	hypothetical protein	-
H4F95_RS26550 (42308)	42308..42403	-	96	WP_000609148.1	DinQ-like type I toxin DqlB	-
H4F95_RS03110 (42904)	42904..43155	+	252	WP_001291964.1	hypothetical protein	-
H4F95_RS03115 (43227)	43227..43379	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (43431)	43431..43491	+	61	NuclAT_0	-	Antitoxin
- (43431)	43431..43491	+	61	NuclAT_0	-	Antitoxin
- (43431)	43431..43491	+	61	NuclAT_0	-	Antitoxin
- (43431)	43431..43491	+	61	NuclAT_0	-	Antitoxin
H4F95_RS03120 (43700)	43700..44074	+	375	WP_223349261.1	hypothetical protein	-
H4F95_RS03125 (44227)	44227..45384	+	1158	Protein_55	IS1380-like element ISEcp1 family transposase	-
H4F95_RS03130 (45440)	45440..46137	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
H4F95_RS03135 (46148)	46148..46456	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
H4F95_RS03140 (46898)	46898..47185	-	288	WP_000074855.1	conjugal transfer protein TraA	-
H4F95_RS26330 (47223)	47223..47381	-	159	Protein_59	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..48292	48292
	-	inside	IScluster/Tn	blaCMY-42	-	39214..45715	6501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T202738 WP_001387489.1 NZ_CP075060:c43379-43227 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T202738 NZ_CP099599:174948-175229 [Pseudomonas siliginis]
ATGACTTATAAGCTTGAGTTTCTGCCGTCTGCGCACAAAGAGTGGAACAAGCTGGGTCATACGTTGCGGGAGCAGTTCAA
AAAGAAGCTGGGTGAGAGGCTGAAGCTTCCCAGAATCCCGGCTGACGCACTCCACGGCATGCCCGACTGCTACAAAATCA
AACTCAAAGCCTCGGGATATCGATTGGTTTACCAAGTCATCGACGAACGAGTCGTGGTTTCCGTGCTAGCGGTTGGCAAG
CGCGAACGCAGCAGCGTTTACGATGCTGCCAGGAACCGTTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT202738 NZ_CP075060:43431-43491 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin