Detailed information of TA system TA202722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4421272..4421874
• Replicon: chromosome
• Accession: NZ_CP075037
• Organism: Salmonella enterica subsp. enterica serovar Give strain CFSAN012622

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3V8XMR3
• Locus tag: GJ28_RS21190
• Protein ID: WP_001159631.1
• Coordinates: 4421563..4421874 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GJ28_RS21185
• Protein ID: WP_000362050.1
• Coordinates: 4421272..4421562 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJ28_RS21170 (4418765)	4418765..4419667	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
GJ28_RS21175 (4419664)	4419664..4420299	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
GJ28_RS21180 (4420296)	4420296..4421225	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
GJ28_RS21185 (4421272)	4421272..4421562	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
GJ28_RS21190 (4421563)	4421563..4421874	-	312	WP_001159631.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
GJ28_RS21195 (4422092)	4422092..4423021	+	930	WP_001127698.1	alpha/beta hydrolase	-
GJ28_RS21200 (4423107)	4423107..4423418	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
GJ28_RS21205 (4423415)	4423415..4423861	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	-
GJ28_RS21210 (4423876)	4423876..4424817	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
GJ28_RS21215 (4424862)	4424862..4425299	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
GJ28_RS21220 (4425296)	4425296..4426168	-	873	WP_000921423.1	virulence factor BrkB family protein	-
GJ28_RS21225 (4426162)	4426162..4426761	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12312.25 Da
• Isoelectric Point: 9.4460

>T202722 WP_001159631.1 NZ_CP075037:c4421874-4421563 [Salmonella enterica subsp. enterica serovar Give]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEVRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T202722 NZ_CP099591:11680-11985 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT202722 WP_000362050.1 NZ_CP075037:c4421562-4421272 [Salmonella enterica subsp. enterica serovar Give]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT202722 NZ_CP099591:11460-11678 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A3V8XMR3