Detailed information of TA system TA202701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13831..14482
• Replicon: plasmid unnamed5
• Accession: NZ_CP074711
• Organism: Acinetobacter baumannii strain ATCC 17978

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V2UF49
• Locus tag: KIP77_RS19980
• Protein ID: WP_000269909.1
• Coordinates: 13831..14187 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KIP77_RS19985
• Protein ID: WP_001140621.1
• Coordinates: 14180..14482 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIP77_RS19950 (KIP77_19815)	8906..9295	+	390	WP_000078281.1	tautomerase family protein	-
KIP77_RS19955 (KIP77_19820)	9478..9621	-	144	WP_001125249.1	hypothetical protein	-
KIP77_RS19960 (KIP77_19825)	9642..10196	-	555	WP_001096621.1	plasmid replication DNA-binding protein	-
KIP77_RS19965 (KIP77_19830)	10189..11145	-	957	WP_000786840.1	replication initiation protein RepM	-
KIP77_RS19970 (KIP77_19835)	12110..12478	+	369	WP_000139283.1	LysR family transcriptional regulator	-
KIP77_RS19975 (KIP77_19840)	12677..12817	+	141	Protein_18	transposase	-
KIP77_RS19980 (KIP77_19845)	13831..14187	+	357	WP_000269909.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIP77_RS19985 (KIP77_19850)	14180..14482	+	303	WP_001140621.1	XRE family transcriptional regulator	Antitoxin
KIP77_RS19990 (KIP77_19855)	14475..14684	+	210	WP_000069472.1	hypothetical protein	-
KIP77_RS19995 (KIP77_19860)	14822..15952	+	1131	WP_000085720.1	DUF262 domain-containing protein	-
KIP77_RS20000 (KIP77_19865)	15959..16672	+	714	WP_061855755.1	HEPN domain-containing protein	-
KIP77_RS20005 (KIP77_19870)	16870..17481	+	612	WP_000571899.1	recombinase family protein	-
KIP77_RS20010 (KIP77_19875)	17697..18215	+	519	WP_000447196.1	tetratricopeptide repeat protein	-
KIP77_RS20015 (KIP77_19880)	18254..18766	+	513	WP_001089572.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38118	38118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13515.49 Da
• Isoelectric Point: 6.4631

>T202701 WP_000269909.1 NZ_CP074711:13831-14187 [Acinetobacter baumannii]
MWTVITTDLFNEWLVQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T202701 NZ_CP099590:714430-714726 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11179.02 Da
• Isoelectric Point: 7.3198

>AT202701 WP_001140621.1 NZ_CP074711:14180-14482 [Acinetobacter baumannii]
MAKTLQELLASRSPESQARIQKMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT202701 NZ_CP099590:714728-715123 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• AlphaFold DB: V2UF49