Detailed information of TA system TA202700

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 423..1074
• Replicon: plasmid unnamed5
• Accession: NZ_CP074711
• Organism: Acinetobacter baumannii strain ATCC 17978

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V2UF49
• Locus tag: KIP77_RS19885
• Protein ID: WP_000269909.1
• Coordinates: 423..779 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KIP77_RS19890
• Protein ID: WP_001140621.1
• Coordinates: 772..1074 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIP77_RS19885 (KIP77_19755)	423..779	+	357	WP_000269909.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIP77_RS19890 (KIP77_19760)	772..1074	+	303	WP_001140621.1	XRE family transcriptional regulator	Antitoxin
KIP77_RS19895 (KIP77_19765)	1067..1276	+	210	WP_000069472.1	hypothetical protein	-
KIP77_RS19900	1413..1883	+	471	WP_229997907.1	DUF262 domain-containing protein	-
KIP77_RS19905	1991..2542	+	552	WP_229997908.1	hypothetical protein	-
KIP77_RS19910 (KIP77_19775)	2549..2848	+	300	WP_229997909.1	hypothetical protein	-
KIP77_RS19915 (KIP77_19780)	2865..3263	+	399	WP_229997910.1	hypothetical protein	-
KIP77_RS19920 (KIP77_19785)	3461..4072	+	612	WP_000571899.1	recombinase family protein	-
KIP77_RS19925 (KIP77_19790)	4288..4806	+	519	WP_000447196.1	tetratricopeptide repeat protein	-
KIP77_RS19930 (KIP77_19795)	4845..5357	+	513	WP_001089572.1	SMI1/KNR4 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38118	38118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13515.49 Da
• Isoelectric Point: 6.4631

>T202700 WP_000269909.1 NZ_CP074711:423-779 [Acinetobacter baumannii]
MWTVITTDLFNEWLVQQDESTQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKGGKKRFYKEMLDIADQQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T202700 NZ_CP099590:648519-648833 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11179.02 Da
• Isoelectric Point: 7.3198

>AT202700 WP_001140621.1 NZ_CP074711:772-1074 [Acinetobacter baumannii]
MAKTLQELLASRSPESQARIQKMADELLLETQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT202700 NZ_CP099590:648830-649246 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: V2UF49