Detailed information of TA system TA202692

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27718..27987
• Replicon: plasmid p702_18_4
• Accession: NZ_CP074705
• Organism: Escherichia coli strain 702/18

Toxin (Protein)

• Gene name: hok
• Uniprot ID: G9G195
• Locus tag: KH969_RS26760
• Protein ID: WP_001323520.1
• Coordinates: 27871..27987 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27718..27783 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH969_RS26725	22764..23012	+	249	WP_071606928.1	hypothetical protein	-
KH969_RS26730	23485..24024	+	540	WP_000290791.1	single-stranded DNA-binding protein	-
KH969_RS26735	24087..24320	+	234	WP_000005990.1	DUF905 family protein	-
KH969_RS26740	24386..26344	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
KH969_RS26745	26399..26833	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
KH969_RS26750	26830..27592	+	763	Protein_36	plasmid SOS inhibition protein A	-
KH969_RS26755	27561..27749	-	189	WP_001299721.1	hypothetical protein	-
-	27561..27785	+	225	NuclAT_0	-	-
-	27561..27785	+	225	NuclAT_0	-	-
-	27561..27785	+	225	NuclAT_0	-	-
-	27561..27785	+	225	NuclAT_0	-	-
-	27718..27783	-	66	-	-	Antitoxin
KH969_RS27420	27771..27920	+	150	Protein_38	plasmid maintenance protein Mok	-
KH969_RS26760	27871..27987	+	117	WP_001323520.1	type I toxin-antitoxin system Hok family toxin	Toxin
KH969_RS27425	28207..28437	+	231	WP_071587244.1	hypothetical protein	-
KH969_RS27430	28435..28608	-	174	Protein_41	hypothetical protein	-
KH969_RS26765	28678..28884	+	207	WP_000547939.1	hypothetical protein	-
KH969_RS26770	28909..29196	+	288	WP_000107535.1	hypothetical protein	-
KH969_RS26775	29318..30139	+	822	WP_001234469.1	DUF932 domain-containing protein	-
KH969_RS26780	30436..31038	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
KH969_RS26785	31359..31742	+	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KH969_RS26790	31929..32618	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4455.23 Da
• Isoelectric Point: 8.5110

>T202692 WP_001323520.1 NZ_CP074705:27871-27987 [Escherichia coli]
VCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 117 bp

>T202692 NZ_CP099588:4024075-4024332 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT202692 NZ_CP074705:c27783-27718 [Escherichia coli]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Toxin

• AlphaFold DB: G9G195

Antitoxin