Detailed information of TA system TA202690

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 37955..38666
• Replicon: plasmid p702_18_3
• Accession: NZ_CP074704
• Organism: Escherichia coli strain 702/18

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: KH969_RS26190
• Protein ID: WP_000162415.1
• Coordinates: 37955..38257 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KH969_RS26195
• Protein ID: WP_032220986.1
• Coordinates: 38328..38666 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH969_RS26165 (KH969_26175)	33638..34543	-	906	WP_053919808.1	recombination-associated protein RdgC	-
KH969_RS26170 (KH969_26180)	34603..35256	-	654	WP_053919809.1	hypothetical protein	-
KH969_RS26175 (KH969_26185)	35253..36233	-	981	WP_000046500.1	hypothetical protein	-
KH969_RS26180 (KH969_26190)	36237..37004	-	768	WP_000203293.1	hypothetical protein	-
KH969_RS26185 (KH969_26195)	37001..37792	-	792	WP_000532307.1	hypothetical protein	-
KH969_RS26190 (KH969_26200)	37955..38257	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KH969_RS26195 (KH969_26205)	38328..38666	+	339	WP_032220986.1	HigA family addiction module antitoxin	Antitoxin
KH969_RS26200 (KH969_26210)	38723..38908	+	186	Protein_40	hypothetical protein	-
KH969_RS26205 (KH969_26215)	38945..39673	+	729	WP_080028515.1	hypothetical protein	-
KH969_RS26210 (KH969_26220)	39707..40900	-	1194	WP_001112629.1	hypothetical protein	-
KH969_RS26215 (KH969_26225)	40893..41222	-	330	WP_000542383.1	hypothetical protein	-
KH969_RS26220 (KH969_26230)	41551..42204	-	654	WP_023908597.1	hypothetical protein	-
KH969_RS26225 (KH969_26235)	42599..43096	+	498	WP_001436217.1	hypothetical protein	-
KH969_RS27390	43117..43509	+	393	WP_000824759.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95167	95167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T202690 WP_000162415.1 NZ_CP074704:37955-38257 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T202690 NZ_CP099588:3670237-3670515 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13068.01 Da
• Isoelectric Point: 6.4754

>AT202690 WP_032220986.1 NZ_CP074704:38328-38666 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNMNKKAA

Nucleotide

• Length: 339 bp

>AT202690 NZ_CP099588:3669974-3670234 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3