Detailed information of TA system TA020269

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47256..47525
• Replicon: plasmid R100
• Accession: NC_002134
• Organism: Shigella flexneri 2b

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HXF40_RS00290
• Protein ID: WP_001372321.1
• Coordinates: 47400..47525 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	47256..47321 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXF40_RS00260 (D742_p2083)	42856..43449	-	594	WP_000428546.1	tetracyline resistance-associated transcriptional repressor TetC	-
HXF40_RS00265 (D742_p2082)	43537..43953	+	417	WP_000275180.1	AraC family transcriptional regulator	-
HXF40_RS00270 (D742_p2015)	43963..45171	-	1209	WP_001339197.1	IS4-like element ISVsa5 family transposase	-
HXF40_RS00275 (D742_p2003)	45281..45868	+	588	Protein_54	hypothetical protein	-
HXF40_RS00280 (D742_p2091)	45937..46371	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HXF40_RS00285 (D742_p2092)	46368..47087	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	47256..47321	+	66	-	-	Antitoxin
HXF40_RS00580	47309..47458	+	150	Protein_57	plasmid maintenance protein Mok	-
HXF40_RS00290 (D742_p2111)	47400..47525	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HXF40_RS00295 (D742_p2002)	47844..48140	-	297	Protein_59	hypothetical protein	-
HXF40_RS00300 (D742_p2001)	48440..48736	+	297	WP_001272251.1	hypothetical protein	-
HXF40_RS00305 (D742_p2116)	48847..49668	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HXF40_RS00310 (D742_p2045)	49965..50567	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HXF40_RS00315 (D742_p2066)	50890..51273	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HXF40_RS00320 (D742_p2069)	51467..52138	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HXF40_RS00325 (D742_p2055)	52275..52502	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / ant(3'')-Ia / catA1 / tet(B)	-	1..94281	94281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T20269 WP_001372321.1 NC_002134:47400-47525 [Shigella flexneri 2b]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T20269 NC_002134:47400-47525 [Shigella flexneri 2b]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT20269 NC_002134:47256-47321 [Shigella flexneri 2b]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin