Detailed information of TA system TA202678

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2529166..2529965
• Replicon: chromosome
• Accession: NZ_CP074701
• Organism: Escherichia coli strain 702/18

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: KH969_RS12240
• Protein ID: WP_000347273.1
• Coordinates: 2529501..2529965 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: KH969_RS12235
• Protein ID: WP_021547164.1
• Coordinates: 2529166..2529501 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH969_RS12220 (2524951)	2524951..2525721	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
KH969_RS12225 (2525737)	2525737..2527071	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
KH969_RS12230 (2527446)	2527446..2529017	+	1572	WP_001273756.1	galactarate dehydratase	-
KH969_RS12235 (2529166)	2529166..2529501	+	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KH969_RS12240 (2529501)	2529501..2529965	+	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KH969_RS12245 (2530020)	2530020..2530829	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
KH969_RS12250 (2531078)	2531078..2532358	+	1281	WP_000681958.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KH969_RS12255 (2532381)	2532381..2532854	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KH969_RS12260 (2532865)	2532865..2533644	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KH969_RS12265 (2533634)	2533634..2534512	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
KH969_RS12270 (2534530)	2534530..2534964	+	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2518683..2529965	11282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T202678 WP_000347273.1 NZ_CP074701:2529501-2529965 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T202678 NZ_CP099588:1780230-1780484 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT202678 WP_021547164.1 NZ_CP074701:2529166-2529501 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT202678 NZ_CP099588:1779982-1780233 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39