Detailed information of TA system TA202648

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110390..110811
• Replicon: plasmid pMEI008_1
• Accession: NZ_CP074681
• Organism: Escherichia coli strain MEI008

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KIM92_RS24105
• Protein ID: WP_096937776.1
• Coordinates: 110390..110515 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	110613..110811 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM92_RS24065 (105455)	105455..105670	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
KIM92_RS24070 (105806)	105806..106453	-	648	WP_134241082.1	conjugal transfer transcriptional regulator TraJ	-
KIM92_RS24075 (106645)	106645..107028	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KIM92_RS24080 (107380)	107380..107970	+	591	WP_166494936.1	transglycosylase SLT domain-containing protein	-
KIM92_RS24085 (108265)	108265..109086	-	822	WP_001234475.1	DUF932 domain-containing protein	-
KIM92_RS24090 (109205)	109205..109492	-	288	WP_000107546.1	hypothetical protein	-
KIM92_RS24095 (109517)	109517..109723	-	207	WP_000547965.1	hypothetical protein	-
KIM92_RS24100 (109793)	109793..110089	+	297	Protein_110	hypothetical protein	-
KIM92_RS24105 (110390)	110390..110515	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
KIM92_RS24110 (110457)	110457..110606	-	150	Protein_112	DUF5431 family protein	-
- (110613)	110613..110811	-	199	NuclAT_0	-	Antitoxin
- (110613)	110613..110811	-	199	NuclAT_0	-	Antitoxin
- (110613)	110613..110811	-	199	NuclAT_0	-	Antitoxin
- (110613)	110613..110811	-	199	NuclAT_0	-	Antitoxin
KIM92_RS24115 (110780)	110780..111542	-	763	Protein_113	plasmid SOS inhibition protein A	-
KIM92_RS24120 (111539)	111539..111973	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
KIM92_RS24125 (112028)	112028..113986	-	1959	WP_029487628.1	ParB/RepB/Spo0J family partition protein	-
KIM92_RS24130 (114052)	114052..114285	-	234	WP_000005990.1	DUF905 family protein	-
KIM92_RS24135 (114342)	114342..114881	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
KIM92_RS24140 (115199)	115199..115708	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..158291	158291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T202648 WP_096937776.1 NZ_CP074681:c110515-110390 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T202648 NZ_CP099556:1061008-1061274 [Aliarcobacter cryaerophilus]
ATGGTAAACTATAAACTTGTTTATACTAAACAAGCTGGAAAAGATGCCAAGAAATTATCTAATTCAGGCTTAAAATCAAA
GGCTTTGGAATTATTAGAGATAATTTCTTTAAATCCATATCAAAATCCCCCGCCTTATGAAAAACTAATTGGTGATTTAA
ATAGTGCAATATCAAGAAGAATAAATATTCAACACAGATTAGTTTATGAAGTTTTAGAAGATATTAAAACTATAAAAGTG
ATACGAATGTGGTCACATTATGAGTAA

Antitoxin

Nucleotide

• Length: 199 bp

>AT202648 NZ_CP074681:c110811-110613 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin