Detailed information of TA system
Overview
TA module
| Type | I | Classification (family/domain) | hok-sok/- |
| Location | 72236..72490 | Replicon | plasmid pMEI008_1 |
| Accession | NZ_CP074681 | ||
| Organism | Escherichia coli strain MEI008 | ||
Toxin (Protein)
| Gene name | srnB | Uniprot ID | G9G1E3 |
| Locus tag | KIM92_RS23875 | Protein ID | WP_001312851.1 |
| Coordinates | 72236..72385 (-) | Length | 50 a.a. |
Antitoxin (RNA)
| Gene name | srnC | ||
| Locus tag | - | ||
| Coordinates | 72429..72490 (+) |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KIM92_RS23845 (67518) | 67518..70523 | + | 3006 | WP_001143760.1 | Tn3-like element Tn3 family transposase | - |
| KIM92_RS23850 (70456) | 70456..70921 | - | 466 | Protein_60 | plasmid replication initiator RepA | - |
| KIM92_RS23855 (70914) | 70914..71396 | - | 483 | WP_001273588.1 | hypothetical protein | - |
| KIM92_RS23860 (71389) | 71389..71436 | - | 48 | WP_229471593.1 | hypothetical protein | - |
| KIM92_RS23865 (71427) | 71427..71678 | + | 252 | WP_223195197.1 | replication protein RepA | - |
| KIM92_RS23870 (71695) | 71695..71952 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| KIM92_RS23875 (72236) | 72236..72385 | - | 150 | WP_001312851.1 | Hok/Gef family protein | Toxin |
| - (72429) | 72429..72490 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (72429) | 72429..72490 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (72429) | 72429..72490 | + | 62 | NuclAT_1 | - | Antitoxin |
| - (72429) | 72429..72490 | + | 62 | NuclAT_1 | - | Antitoxin |
| KIM92_RS23880 (72746) | 72746..72820 | - | 75 | Protein_66 | endonuclease | - |
| KIM92_RS23885 (73066) | 73066..73278 | - | 213 | WP_001299730.1 | ANR family transcriptional regulator | - |
| KIM92_RS23890 (73414) | 73414..73974 | - | 561 | WP_000139315.1 | fertility inhibition protein FinO | - |
| KIM92_RS23895 (74077) | 74077..74937 | - | 861 | WP_000704513.1 | alpha/beta hydrolase | - |
| KIM92_RS23900 (74996) | 74996..75742 | - | 747 | WP_000205749.1 | conjugal transfer pilus acetylase TraX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaTEM-1B / sitABCD | iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA | 1..158291 | 158291 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 50 a.a. Molecular weight: 5542.67 Da Isoelectric Point: 8.7678
>T202645 WP_001312851.1 NZ_CP074681:c72385-72236 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK
Download Length: 150 bp
>T202645 NZ_CP099547:c1647118-1646864 [Arcanobacterium pinnipediorum]
GTGAAATATGTCTGGGATGAGAACGCCTGGCAAGACTACCTATGGTGGCAAGCTCACGACCGGAAGATGCTGAAGCGAAT
CAATCTCTTGCTTAAAGATATTTCGCGCAACGGAAACGAAGGTATCGGGAAGCCGGAACCATTGAAATATGAGCTTGCCG
GATATTGGTCGCGCAGGATCTCTGATGAACATCGCCTCGTTTTTAAGTACACCGAGACGGCAGTATATATAGCTGCTTGC
CGATACCATTATTAG
GTGAAATATGTCTGGGATGAGAACGCCTGGCAAGACTACCTATGGTGGCAAGCTCACGACCGGAAGATGCTGAAGCGAAT
CAATCTCTTGCTTAAAGATATTTCGCGCAACGGAAACGAAGGTATCGGGAAGCCGGAACCATTGAAATATGAGCTTGCCG
GATATTGGTCGCGCAGGATCTCTGATGAACATCGCCTCGTTTTTAAGTACACCGAGACGGCAGTATATATAGCTGCTTGC
CGATACCATTATTAG
Antitoxin
Download Length: 62 bp
>AT202645 NZ_CP074681:72429-72490 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
