Detailed information of TA system TA202644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4672420..4673022
• Replicon: chromosome
• Accession: NZ_CP074680
• Organism: Escherichia coli strain MEI008

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KIM92_RS22550
• Protein ID: WP_000897305.1
• Coordinates: 4672711..4673022 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KIM92_RS22545
• Protein ID: WP_000356397.1
• Coordinates: 4672420..4672710 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM92_RS22520 (4668345)	4668345..4669247	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KIM92_RS22525 (4669244)	4669244..4669879	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KIM92_RS22530 (4669876)	4669876..4670805	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KIM92_RS22535 (4671135)	4671135..4671377	-	243	WP_001087409.1	protein YiiF	-
KIM92_RS22540 (4671597)	4671597..4671815	-	219	WP_001315930.1	CopG family transcriptional regulator	-
KIM92_RS22545 (4672420)	4672420..4672710	-	291	WP_000356397.1	NadS family protein	Antitoxin
KIM92_RS22550 (4672711)	4672711..4673022	-	312	WP_000897305.1	hypothetical protein	Toxin
KIM92_RS22555 (4673251)	4673251..4674159	+	909	WP_001162704.1	alpha/beta hydrolase	-
KIM92_RS22560 (4674223)	4674223..4675164	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KIM92_RS22565 (4675209)	4675209..4675646	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KIM92_RS22570 (4675643)	4675643..4676515	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KIM92_RS22575 (4676509)	4676509..4677108	-	600	WP_001295269.1	glucose-1-phosphatase	-
KIM92_RS22580 (4677207)	4677207..4677992	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T202644 WP_000897305.1 NZ_CP074680:c4673022-4672711 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T202644 NZ_CP099544:755657-755917 [Photobacterium damselae]
ATGATGATTAATTTCACGCCTAACGCTTGGGATGATTACTTATACTGGCAATCTCAAGATAAGAAGACTTTAAAACGAAT
CAATAAACTGATTGAGAATTGTGTTAGAACGCCGTTCGAAGGAATTGGAAAGCCTGAACCATTGAAAGATAACTTATCAG
GCTATTGGTCGCGACGCATTGATGATACACATAGATTAGTCTATAAAGCCACTCAAGATACTTTAGTCATTGTTGCTGTT
CGGTTTCACTACCAAAAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT202644 WP_000356397.1 NZ_CP074680:c4672710-4672420 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT202644 NZ_CP099544:755407-755667 [Photobacterium damselae]
ATGACCGTTTTGAGTTTTTCTGAAGTTCGAGGCAATTTTAAAAAAGTATGCGATCAAGTCGTTGATGATTGCGATGCAGT
AGTTATCCATCGACGTGATGCAGATAATGTCGTGCTTATGTCTGAATCTGAGTTTAATAGTTGGAAGGAAACGATGTATC
TCATGTCTAATCCTGTAAATGCTGCAAACTTACTTGAATCAATAAAACAAGCTGAATGTGGTAATGTACAGACACATGAG
CTACTAAGCCATGATGATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7