Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3772463..3773004 Replicon chromosome
Accession NZ_CP074680
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag KIM92_RS18360 Protein ID WP_000615976.1
Coordinates 3772726..3773004 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag KIM92_RS18355 Protein ID WP_000729704.1
Coordinates 3772463..3772723 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS18330 (3768246) 3768246..3769031 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
KIM92_RS18335 (3769003) 3769003..3770715 + 1713 Protein_3590 flagellar biosynthesis protein FlhA -
KIM92_RS18340 (3770820) 3770820..3771098 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
KIM92_RS18345 (3771091) 3771091..3771447 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
KIM92_RS18350 (3771504) 3771504..3772277 - 774 WP_000543895.1 C40 family peptidase -
KIM92_RS18355 (3772463) 3772463..3772723 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
KIM92_RS18360 (3772726) 3772726..3773004 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
KIM92_RS18365 (3773160) 3773160..3773900 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
KIM92_RS18370 (3773871) 3773871..3774638 - 768 WP_000333380.1 class II glutamine amidotransferase -
KIM92_RS18375 (3774844) 3774844..3775422 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR 3771091..3788990 17899


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T202638 WP_000615976.1 NZ_CP074680:3772726-3773004 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T202638 NZ_CP099542:c86235-85837 [Pantoea ananatis]
ATGCTCGACACCTGCATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCGGTACTGAAGCGCCTTGAGCAGGCGGTGCT
GCGCGGCCACCGTATCGTGGTCTCGGCCATCACCTATGCCGAAATGCGTTTTGGTGCCTCCGGCCCGAAGGCCTCGCCCC
GCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGCCTCGACGCCGTCCTGCCCTGGGACCGTGCCGCGGTGGATGCGACC
ACGGAGATCAAAGTTTCGCTGCGGCTGGCCGGCACGCCAATCGGCCCGAACGACACGGCCATCGCCGGGCATGCAATCAC
GGTCGGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGCGCGGGTGCCGGGCCTGGCCCTGGAAGACTGGGTATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT202638 WP_000729704.1 NZ_CP074680:3772463-3772723 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT202638 NZ_CP099542:c86489-86259 [Pantoea ananatis]
ATGAGAACTGTATCGATATTTAAAAACGGCAACAACCGTGCCATCCGCCTGCCCCGCGATCTGGATTTTGACGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTCG
AAAAAGCCGATCCGCACTTTATGGCGGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGTTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References