Detailed information of TA system TA202608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3691423..3692020
• Replicon: chromosome
• Accession: NZ_CP074677
• Organism: Klebsiella pneumoniae strain MEI009

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: KIM99_RS18040
• Protein ID: WP_004893639.1
• Coordinates: 3691703..3692020 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KIM99_RS18035
• Protein ID: WP_004142561.1
• Coordinates: 3691423..3691710 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM99_RS18010 (3686693)	3686693..3687808	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
KIM99_RS18015 (3687988)	3687988..3688569	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
KIM99_RS18020 (3688569)	3688569..3688937	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
KIM99_RS18025 (3689057)	3689057..3689710	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
KIM99_RS18035 (3691423)	3691423..3691710	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KIM99_RS18040 (3691703)	3691703..3692020	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIM99_RS18045 (3692205)	3692205..3693248	-	1044	WP_009308098.1	DUF2157 domain-containing protein	-
KIM99_RS18050 (3693914)	3693914..3694780	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
KIM99_RS18055 (3694889)	3694889..3696316	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T202608 WP_004893639.1 NZ_CP074677:c3692020-3691703 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T202608 NZ_CP099523:107574-108017 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT202608 WP_004142561.1 NZ_CP074677:c3691710-3691423 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT202608 NZ_CP099523:107347-107577 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1