Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 327608..328194 Replicon chromosome
Accession NZ_CP074677
Organism Klebsiella pneumoniae strain MEI009

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KIM99_RS01525 Protein ID WP_002920800.1
Coordinates 327826..328194 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KIM99_RS01520 Protein ID WP_004174006.1
Coordinates 327608..327829 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM99_RS01500 (323765) 323765..324691 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KIM99_RS01505 (324688) 324688..325965 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KIM99_RS01510 (325962) 325962..326729 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KIM99_RS01515 (326731) 326731..327444 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KIM99_RS01520 (327608) 327608..327829 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KIM99_RS01525 (327826) 327826..328194 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KIM99_RS01530 (328467) 328467..329783 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KIM99_RS01535 (329890) 329890..330777 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KIM99_RS01540 (330774) 330774..331619 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KIM99_RS01545 (331621) 331621..332691 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 324688..333428 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T202594 WP_002920800.1 NZ_CP074677:327826-328194 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T202594 NZ_CP099513:c4739067-4738744 [Klebsiella sp. ZYC-1]
ATGAATACTTTACCTGCAATAAACCAGCGGGCTGTTCAGCCATATCCATCGCCCGTGGTTGTCTGGCAAATGCTGCTTGC
TCGGCTACTTGAGCAACACTACGGCCTGACGTTAAATGACACGCCGTTCAGCGATGAAGCTGTTATTAAGGGACATATCG
AAGCTGGCATCACGCTTGTCGATGCCGTTAATTTTCTGGTGGAGAAGTACGAGTTGGTCCGTGTCGACCAAAGGGGATTT
AGCTGGCAGGAGCAAGCGCCTTACCTTACGATTGTTGATATCATGAGGGCTCGTCGCTATCTTGGTATCACTAACCGTAG
CTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT202594 WP_004174006.1 NZ_CP074677:327608-327829 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT202594 NZ_CP099513:c4739453-4739103 [Klebsiella sp. ZYC-1]
ATGTCGAATAAGACACTGACAGCGAATGACGATACTGCTGAGCCCTGGTGGGGCTTGAGTCGTAACGTCATCCCTTGTTT
TGGCGCACGTATGGTCCAGGAAGGTAATCGCCTGCATTACCTGGCTGACCGCGCCAGCATAACAGGTCAGTTCAATGAAG
CGGATTTACTCCATCTTGACCAGGCATTCCCGGTACTGCTGAAGCAGGTGGAGCTCATGCTTACCAGCGGCGAACTAAAT
CCCCGACACCAGCATTGCGTTACGCTCTATGCAAAGGGGCTGACCTGCGAAGCTGATACGCTGGGCTCCTGCGGTTACGT
TTATATCGCCATTTATCCCACCCAAAGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References