Detailed information of TA system TA202590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 242603..243204
• Replicon: chromosome
• Accession: NZ_CP074676
• Organism: Pseudomonas qingdaonensis strain S-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1Y3L894
• Locus tag: KH389_RS01115
• Protein ID: WP_043861628.1
• Coordinates: 242890..243204 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KH389_RS01110
• Protein ID: WP_058541716.1
• Coordinates: 242603..242890 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH389_RS01085 (KH389_01085)	238017..238499	-	483	WP_054915912.1	ATP-dependent zinc protease	-
KH389_RS01090 (KH389_01090)	238609..239010	+	402	WP_043861624.1	S4 domain-containing protein	-
KH389_RS01095 (KH389_01095)	239007..239810	-	804	WP_058541684.1	phosphatase PAP2 family protein	-
KH389_RS01100 (KH389_01100)	239916..240818	+	903	WP_043861626.1	Hsp33 family molecular chaperone HslO	-
KH389_RS01105 (KH389_01105)	240998..242539	+	1542	WP_043861627.1	phosphoenolpyruvate carboxykinase	-
KH389_RS01110 (KH389_01110)	242603..242890	-	288	WP_058541716.1	helix-turn-helix domain-containing protein	Antitoxin
KH389_RS01115 (KH389_01115)	242890..243204	-	315	WP_043861628.1	hypothetical protein	Toxin
KH389_RS01120 (KH389_01120)	243384..243812	+	429	WP_213606703.1	thioesterase family protein	-
KH389_RS01125 (KH389_01125)	243825..244481	-	657	WP_213606705.1	response regulator transcription factor	-
KH389_RS01130 (KH389_01130)	244521..245588	-	1068	WP_213606707.1	chemotaxis response regulator protein-glutamate methylesterase	-
KH389_RS01135 (KH389_01135)	245585..246397	-	813	WP_043861632.1	protein-glutamate O-methyltransferase CheR	-
KH389_RS01140 (KH389_01140)	246398..246910	-	513	WP_043861633.1	chemotaxis protein CheW	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-88)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11751.52 Da
• Isoelectric Point: 9.8352

>T202590 WP_043861628.1 NZ_CP074676:c243204-242890 [Pseudomonas qingdaonensis]
MLFIETSVFTSDVSSHLDDHEYLSLQVYLAEHPEAGDLIEDTGGLRKIRWAAKGKGKSGGVRVIYYYAVSASQIRLLLIY
RKGIHDTLTKKQKAVLRSLNQGWK

Nucleotide

• Length: 315 bp

>T202590 NZ_CP099513:1095801-1096058 [Klebsiella sp. ZYC-1]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACGTTCGCGATGCATC
AGGCCGCGTCACTTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTATCGCATCAATACCACCGGGCTGAATAATGTCCGCGCA
CAGCTGGATAATCGCTAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10796.31 Da
• Isoelectric Point: 7.1697

>AT202590 WP_058541716.1 NZ_CP074676:c242890-242603 [Pseudomonas qingdaonensis]
MDRNLFGRLVDSMTEMAEIDRGERKPSREFQVDAMRVRAIREATGLTQERFAQCIDVPLGTLRNWEQGRRAPEGPARALL
RAIHNDPEHVLAALA

Nucleotide

• Length: 288 bp

>AT202590 NZ_CP099513:1096069-1096620 [Klebsiella sp. ZYC-1]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCAATGACATTCTCCATGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAAAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTTGCGGTGGTGCCGGAATACCAGGGCGCGGGCGTTGGCGGAGGGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCCATGGGAAGCCAGACGGTATTTGTTCTGGGCCACGCCACTTACTACCCCAGGCATGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCCATTCCCGAGGCGCTCAAAGCCTGCTGGATGCTGCAGCCGCTTTCATCT
CAGCCGCTGGGACGAACGGGTCAAATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A1Y3L894