Detailed information of TA system TA202562

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4479350..4480104
• Replicon: chromosome
• Accession: NZ_CP074661
• Organism: Salmonella enterica subsp. enterica serovar Enteritidis strain CFSAN008104

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5RFE2
• Locus tag: A9D71_RS21615
• Protein ID: WP_000558168.1
• Coordinates: 4479350..4479661 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: A9D71_RS21620
• Protein ID: WP_001259009.1
• Coordinates: 4479658..4480104 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9D71_RS21585 (4475008)	4475008..4475910	+	903	WP_000331364.1	formate dehydrogenase subunit beta	-
A9D71_RS21590 (4475907)	4475907..4476542	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
A9D71_RS21595 (4476539)	4476539..4477468	+	930	WP_000027736.1	formate dehydrogenase accessory protein FdhE	-
A9D71_RS21600 (4477515)	4477515..4477805	-	291	WP_000362050.1	DNA-binding transcriptional regulator	-
A9D71_RS21605 (4477806)	4477806..4478117	-	312	WP_001159635.1	cytotoxic translational repressor of toxin-antitoxin stability system	-
A9D71_RS21610 (4478335)	4478335..4479264	+	930	WP_001127705.1	alpha/beta hydrolase	-
A9D71_RS21615 (4479350)	4479350..4479661	+	312	WP_000558168.1	type II toxin-antitoxin system HigB family toxin	Toxin
A9D71_RS21620 (4479658)	4479658..4480104	+	447	WP_001259009.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
A9D71_RS21625 (4480119)	4480119..4481060	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
A9D71_RS21630 (4481105)	4481105..4481542	-	438	WP_000560968.1	D-aminoacyl-tRNA deacylase	-
A9D71_RS21635 (4481539)	4481539..4482411	-	873	WP_000921423.1	virulence factor BrkB family protein	-
A9D71_RS21640 (4482405)	4482405..4483004	-	600	WP_000965695.1	glucose-1-phosphatase	-
A9D71_RS21645 (4483195)	4483195..4483998	-	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
A9D71_RS21650 (4484032)	4484032..4484928	-	897	WP_001520529.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12324.26 Da
• Isoelectric Point: 9.3143

>T202562 WP_000558168.1 NZ_CP074661:4479350-4479661 [Salmonella enterica subsp. enterica serovar Enteritidis]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTLTQPEEMKRYIPSLDNFKHRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYDRGNKE

Nucleotide

• Length: 312 bp

>T202562 NZ_CP099504:2861494-2861745 [Staphylococcus aureus]
ATGAATAACCGCGAACAAATTGAACAGTCCGTTATAAGTGCTAGTGCGTATAACGGCAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTAAGGGTTTACCTAATGCTATGCAAGATGCAC
TCAAAGAAGATATTGGTCTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16720.06 Da
• Isoelectric Point: 6.6451

>AT202562 WP_001259009.1 NZ_CP074661:4479658-4480104 [Salmonella enterica subsp. enterica serovar Enteritidis]
MRTHRQMDATSAKKIVDTFSDAVKSVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT202562 NZ_CP099504:2861735-2861908 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAAGTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA

Structures

Toxin

• AlphaFold DB: A0A1R2S541