Detailed information of TA system TA202497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14550..15201
• Replicon: plasmid unnamed
• Accession: NZ_CP074586
• Organism: Acinetobacter baumannii strain ATCC 19606

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: KIH05_RS19155
• Protein ID: WP_000269904.1
• Coordinates: 14845..15201 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KIH05_RS19150
• Protein ID: WP_001129974.1
• Coordinates: 14550..14852 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIH05_RS19115 (KIH05_19055)	10589..11350	+	762	WP_000425105.1	hypothetical protein	-
KIH05_RS19120 (KIH05_19060)	11580..11807	+	228	WP_000555568.1	hypothetical protein	-
KIH05_RS19125 (KIH05_19065)	12040..12471	-	432	WP_000042163.1	MarR family transcriptional regulator	-
KIH05_RS19130 (KIH05_19070)	12478..12906	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
KIH05_RS19135 (KIH05_19075)	13104..13616	-	513	WP_001089573.1	SMI1/KNR4 family protein	-
KIH05_RS19140 (KIH05_19080)	13655..14173	-	519	WP_000447196.1	tetratricopeptide repeat protein	-
KIH05_RS19145 (KIH05_19085)	14348..14557	-	210	WP_000069474.1	hypothetical protein	-
KIH05_RS19150 (KIH05_19090)	14550..14852	-	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
KIH05_RS19155 (KIH05_19095)	14845..15201	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIH05_RS19160	15743..16078	-	336	WP_001061698.1	hypothetical protein	-
KIH05_RS19165	16197..16331	+	135	Protein_25	cysteine hydrolase	-
KIH05_RS19170 (KIH05_19100)	16527..17044	-	518	Protein_26	plasmid replication DNA-binding protein	-
KIH05_RS19175 (KIH05_19105)	17122..18057	-	936	WP_001292329.1	replication initiation protein RepM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18598	18598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T202497 WP_000269904.1 NZ_CP074586:c15201-14845 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T202497 NZ_CP099464:4091831-4092022 [Dolichospermum heterosporum TAC447]
ATGAAAGTACGAGCAGTAATTCAGCGACTAGAAGCGGATGGTTGGTATTTAGCCAGAACTAGAGGAAGTCACCGGCAGTT
TAAACATCCTGATAAACTTGGTATAGTTACAGTTTCCGGTAAACCCAATATTGACGTACCCATTGGTACACTAAAAAGTA
TATGGAAACAAGCACAATTGGAGGAAGAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT202497 WP_001129974.1 NZ_CP074586:c14852-14550 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT202497 NZ_CP099464:4092022-4092234 [Dolichospermum heterosporum TAC447]
ATGCGTTATACAGTAGTAATTGAAAAGGGAAATAGCAGTTATGGTGCTTATGTTCCCGACTTACCCGGTTGTATAGCTGT
AGCTGAAACATTAGTAGAAGTTCAACAAATGATTGTTGAAGCAATAGAATTTCATATAGAAGGTTTAATAGAGTCAGGTT
TACCAATTCCCCAACCTACAAGTATCGCCCAAGAAGTTGAAGTTTTGATTTAA

Structures

Toxin

• AlphaFold DB: N8YFZ1