Detailed information of TA system TA202496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5010..5661
• Replicon: plasmid unnamed
• Accession: NZ_CP074586
• Organism: Acinetobacter baumannii strain ATCC 19606

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8YFZ1
• Locus tag: KIH05_RS19085
• Protein ID: WP_000269904.1
• Coordinates: 5305..5661 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KIH05_RS19080
• Protein ID: WP_001129974.1
• Coordinates: 5010..5312 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIH05_RS19040 (KIH05_18990)	37..744	-	708	WP_230070449.1	MobA/MobL family protein	-
KIH05_RS19045 (KIH05_18995)	1049..1810	+	762	WP_000425105.1	hypothetical protein	-
KIH05_RS19050 (KIH05_19000)	2040..2267	+	228	WP_000555568.1	hypothetical protein	-
KIH05_RS19055 (KIH05_19005)	2500..2931	-	432	WP_000042163.1	MarR family transcriptional regulator	-
KIH05_RS19060 (KIH05_19010)	2938..3366	-	429	WP_000105988.1	organic hydroperoxide resistance protein	-
KIH05_RS19065 (KIH05_19015)	3564..4076	-	513	WP_001089573.1	SMI1/KNR4 family protein	-
KIH05_RS19070 (KIH05_19020)	4115..4633	-	519	WP_000447196.1	tetratricopeptide repeat protein	-
KIH05_RS19075 (KIH05_19025)	4808..5017	-	210	WP_000069474.1	hypothetical protein	-
KIH05_RS19080 (KIH05_19030)	5010..5312	-	303	WP_001129974.1	XRE family transcriptional regulator	Antitoxin
KIH05_RS19085 (KIH05_19035)	5305..5661	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIH05_RS19090	6203..6538	-	336	WP_001061698.1	hypothetical protein	-
KIH05_RS19095	6657..6791	+	135	Protein_11	cysteine hydrolase	-
KIH05_RS19100 (KIH05_19040)	6987..7505	-	519	WP_000839337.1	plasmid replication DNA-binding protein	-
KIH05_RS19105 (KIH05_19045)	7583..8518	-	936	WP_001292329.1	replication initiation protein RepM	-
KIH05_RS19110 (KIH05_19050)	9115..10284	-	1170	WP_002134206.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18598	18598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13488.42 Da
• Isoelectric Point: 5.7104

>T202496 WP_000269904.1 NZ_CP074586:c5661-5305 [Acinetobacter baumannii]
MWTVITTDLFNEWLEQQDEATQEKVLAALVVLQQQGPSLGRPLVDTVYDSKFTNMKELRVQHRGKPLRAFFAFDPLRQAI
VLCIGDKSNKKRFYTEMLAIADEQYALHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T202496 NZ_CP099464:c3854825-3854490 [Dolichospermum heterosporum TAC447]
ATGGTAGATTACGTCCCCCGTTATGGGGATCTTGTCTGGCTAAACTTTGATCCCCAATCAGGTCACGAGCAAATGGGGCA
ACGTCCCGCACTAGTGTTAAGTCATACAGAGTTTAACCAGCAACGAGGTTTTATTTTTGTTTGTCCTATCAGTAATACTA
AGCGTAAAAATCCTTTCTACGTTGAGATTCCAGATGATCAACCTATTAAAGGCGTAGTGATGTGCGATCAGCTGCGTTCA
TTGGATTATCGCTCAAGAAAGGCAAAACTGATCTGTATTTGCCCTGAATGGTTATTAGCAGAAGTGCTTGGTCGCATTCA
TCCTATTCTTTTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11150.97 Da
• Isoelectric Point: 7.3198

>AT202496 WP_001129974.1 NZ_CP074586:c5312-5010 [Acinetobacter baumannii]
MAKSLQELLASRSPESQARIQKMADELLLESQLHLIREELEISQKELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT202496 NZ_CP099464:c3855058-3854819 [Dolichospermum heterosporum TAC447]
ATGAAATCTCAAATTGGTCGTTGGGGAAATTCTTTAGCCTTGAGAATCCCCAAGTATATTGCAGATGAATTGGCTTTATC
AATCAATGATGAAGTTGACTGCCGTATTGAGCAAGGGCAACTCATGGTAAGACCAGTTCAAAAATTTCCCAAATACACCC
TTTCACAACTCCTTAGCCAAGATTTAGAGCCAGAAGCGGAGATTGACTGGGGTAAACCAACAGGTAATGAAGAATGGTAG

Structures

Toxin

• AlphaFold DB: N8YFZ1