Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3284818..3285464 Replicon chromosome
Accession NZ_CP074580
Organism Klebsiella pneumoniae strain B30155

Toxin (Protein)


Gene name higB Uniprot ID A0A483PC41
Locus tag KH752_RS15625 Protein ID WP_004216295.1
Coordinates 3284818..3285165 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag KH752_RS15630 Protein ID WP_002920557.1
Coordinates 3285165..3285464 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH752_RS15615 (KH752_15615) 3280744..3282177 + 1434 WP_004889438.1 glycogen synthase GlgA -
KH752_RS15620 (KH752_15620) 3282195..3284642 + 2448 WP_134919826.1 glycogen phosphorylase -
KH752_RS15625 (KH752_15625) 3284818..3285165 + 348 WP_004216295.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KH752_RS15630 (KH752_15630) 3285165..3285464 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
KH752_RS15635 (KH752_15635) 3285527..3287035 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
KH752_RS15640 (KH752_15640) 3287240..3287569 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
KH752_RS15645 (KH752_15645) 3287620..3288450 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
KH752_RS15650 (KH752_15650) 3288500..3289258 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13606.61 Da        Isoelectric Point: 5.6749

>T202466 WP_004216295.1 NZ_CP074580:3284818-3285165 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCADNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T202466 NZ_CP099413:741607-742092 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT202466 WP_002920557.1 NZ_CP074580:3285165-3285464 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT202466 NZ_CP099413:741317-741610 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483PC41

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References