Detailed information of TA system TA202460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 1488033..1488630
• Replicon: chromosome
• Accession: NZ_CP074580
• Organism: Klebsiella pneumoniae strain B30155

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: KH752_RS07125
• Protein ID: WP_004893639.1
• Coordinates: 1488313..1488630 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: KH752_RS07120
• Protein ID: WP_004142561.1
• Coordinates: 1488033..1488320 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH752_RS07090 (KH752_07090)	1484113..1484361	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
KH752_RS07095 (KH752_07095)	1484379..1484720	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
KH752_RS07100 (KH752_07100)	1484751..1485866	-	1116	WP_012737592.1	MBL fold metallo-hydrolase	-
KH752_RS07105 (KH752_07105)	1486046..1486627	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
KH752_RS07110 (KH752_07110)	1486627..1486995	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
KH752_RS07115 (KH752_07115)	1487115..1487768	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
KH752_RS07120 (KH752_07120)	1488033..1488320	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
KH752_RS07125 (KH752_07125)	1488313..1488630	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KH752_RS07130 (KH752_07130)	1488815..1489858	-	1044	WP_004893645.1	DUF2157 domain-containing protein	-
KH752_RS07135 (KH752_07135)	1490524..1491390	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
KH752_RS07140 (KH752_07140)	1491499..1492926	+	1428	WP_004176980.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T202460 WP_004893639.1 NZ_CP074580:c1488630-1488313 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T202460 NZ_CP099402:c425028-424738 [Rhizobium sp. Pop5]
ATGCCACAAGTAATCTTCTCCCCGGCTGCAATCCGTGACCTTGAACGATTGCGCGAGTTCCTGCGGCCGAAAAATCCATC
GGCCGCCAAACGGGCAGGCGAAACGATCCTTAAATCTGTGCGGGCCCTCGGCATGAATCCGTATATGGGACGTCTCGTCG
AGGATTTGCCCGAGCAATACCGGGAATGGCTTATCGATTTCGGAGATAGCGGGGACGTGGTTCGCTACCATATCGATGGA
GACGCTTTGACGATCCTGGCAGTACGGCACCAGAGGGACGCAGGGTTCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT202460 WP_004142561.1 NZ_CP074580:c1488320-1488033 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT202460 NZ_CP099402:c425294-425016 [Rhizobium sp. Pop5]
ATGGCATCGCCGACGTCGCTCAAACTCGATGATGAGCTGAAGGGCCGTGTCCAGCAATTGGCCGAAGTCCGCCGCCGCTC
GTCTCACTGGATCATGCGGGAAGCCATTGCGCAATATGTGGAGCGTGAGGAGAAGCGCGAGGCGCTGCGGCAGGAGACGC
TCAATGCCTGGAACGAATTTCAGGCGACGGGTCTGCACGTGACCGGTGATCAAGTGGAGAATTGGCTTTCGACATGGGGA
ACTGACGAAGAGCTGCCCGCGCCCGAATGCCACAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1