Detailed information of TA system TA202444

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26918..27344
• Replicon: plasmid pCS18F-NDM-Fos
• Accession: NZ_CP074577
• Organism: Escherichia coli strain CS18F

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KHZ70_RS23885
• Protein ID: WP_096937776.1
• Coordinates: 26918..27043 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27120..27344 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHZ70_RS23855 (22091)	22091..22492	-	402	WP_001369361.1	conjugal transfer relaxosome DNA-bindin protein TraY	-
KHZ70_RS23860 (22585)	22585..23274	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
KHZ70_RS23865 (23461)	23461..23844	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
KHZ70_RS23870 (24165)	24165..24767	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
KHZ70_RS23875 (25064)	25064..25885	-	822	WP_001234487.1	DUF932 domain-containing protein	-
KHZ70_RS23880 (25996)	25996..26292	-	297	WP_001272245.1	hypothetical protein	-
KHZ70_RS25015 (26344)	26344..26617	+	274	Protein_35	hypothetical protein	-
KHZ70_RS23885 (26918)	26918..27043	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
KHZ70_RS25020 (26985)	26985..27134	-	150	Protein_37	plasmid maintenance protein Mok	-
- (27120)	27120..27344	-	225	NuclAT_0	-	Antitoxin
- (27120)	27120..27344	-	225	NuclAT_0	-	Antitoxin
- (27120)	27120..27344	-	225	NuclAT_0	-	Antitoxin
- (27120)	27120..27344	-	225	NuclAT_0	-	Antitoxin
KHZ70_RS23890 (27313)	27313..28075	-	763	Protein_38	plasmid SOS inhibition protein A	-
KHZ70_RS23895 (28072)	28072..28506	-	435	WP_000845962.1	conjugation system SOS inhibitor PsiB	-
KHZ70_RS23900 (28575)	28575..30539	-	1965	WP_068862930.1	ParB/RepB/Spo0J family partition protein	-
KHZ70_RS23905 (30603)	30603..30836	-	234	WP_000005988.1	DUF905 family protein	-
KHZ70_RS23910 (30934)	30934..31431	-	498	Protein_42	single-stranded DNA-binding protein	-
KHZ70_RS23915 (31732)	31732..32157	+	426	WP_015387398.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / mph(A) / aph(3')-Ia / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / fosA3	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..189141	189141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T202444 WP_096937776.1 NZ_CP074577:c27043-26918 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T202444 NZ_CP099394:1767697-1767882 [Paraburkholderia bryophila]
ATGAAGTACAGCGAGTTTAGGAAGTGGCTGAGGAAGCAAGGTGCGACCTTCGAGCGGCACAGAAGCGGTTCAATTCACTA
TCGGGTCACGTTGAACGGCAAGACGACCATTTTTCCCGATCACGGAGCCAGGGAAATGGGTACCCACCTTGTCGAAGCGA
TCAAGAATCAGTTGGGTATCCAATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT202444 NZ_CP074577:c27344-27120 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGATTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin