Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4220574..4221176 Replicon chromosome
Accession NZ_CP074576
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag KHZ70_RS20415 Protein ID WP_000897305.1
Coordinates 4220865..4221176 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KHZ70_RS20410 Protein ID WP_000356397.1
Coordinates 4220574..4220864 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS20390 (4217076) 4217076..4217978 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
KHZ70_RS20395 (4217975) 4217975..4218610 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
KHZ70_RS20400 (4218607) 4218607..4219536 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
KHZ70_RS20405 (4219751) 4219751..4219969 - 219 WP_001295676.1 CopG family transcriptional regulator -
KHZ70_RS20410 (4220574) 4220574..4220864 - 291 WP_000356397.1 NadS family protein Antitoxin
KHZ70_RS20415 (4220865) 4220865..4221176 - 312 WP_000897305.1 hypothetical protein Toxin
KHZ70_RS20420 (4221405) 4221405..4222313 + 909 WP_001318161.1 alpha/beta hydrolase -
KHZ70_RS20425 (4222377) 4222377..4223318 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
KHZ70_RS20430 (4223363) 4223363..4223800 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
KHZ70_RS20435 (4223797) 4223797..4224669 - 873 WP_000920762.1 virulence factor BrkB family protein -
KHZ70_RS20440 (4224663) 4224663..4225262 - 600 WP_001315111.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T202438 WP_000897305.1 NZ_CP074576:c4221176-4220865 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T202438 NZ_CP099390:2508232-2508420 [Citrobacter braakii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTAGCTTACAGTCATCATCAATATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT202438 WP_000356397.1 NZ_CP074576:c4220864-4220574 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT202438 NZ_CP099390:2508452-2508868 [Citrobacter braakii]
ATGCGTTATCCCGTTAATCTAATCCCCGCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
TCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCGCTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGAATCGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACGATGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References