Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 140566..141365 Replicon chromosome
Accession NZ_CP074576
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KHZ70_RS00700 Protein ID WP_000347251.1
Coordinates 140566..141030 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KHZ70_RS00705 Protein ID WP_001296435.1
Coordinates 141030..141365 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS00670 (135567) 135567..136001 - 435 WP_000948823.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KHZ70_RS00675 (136019) 136019..136897 - 879 WP_113415970.1 PTS N-acetylgalactosamine transporter subunit IID -
KHZ70_RS00680 (136887) 136887..137666 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KHZ70_RS00685 (137677) 137677..138150 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KHZ70_RS00690 (138173) 138173..139453 - 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
KHZ70_RS00695 (139702) 139702..140511 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KHZ70_RS00700 (140566) 140566..141030 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KHZ70_RS00705 (141030) 141030..141365 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KHZ70_RS00710 (141514) 141514..143085 - 1572 WP_001273941.1 galactarate dehydratase -
KHZ70_RS00715 (143460) 143460..144794 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
KHZ70_RS00720 (144810) 144810..145580 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T202416 WP_000347251.1 NZ_CP074576:c141030-140566 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T202416 NZ_CP099387:c2855042-2854939 [Citrobacter braakii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT202416 WP_001296435.1 NZ_CP074576:c141365-141030 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT202416 NZ_CP099387:2854934-2855077 [Citrobacter braakii]
CACATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTT
GGCATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References