Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3984999..3985596 Replicon chromosome
Accession NZ_CP074528
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag KHV96_RS19575 Protein ID WP_004142563.1
Coordinates 3985279..3985596 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag KHV96_RS19570 Protein ID WP_004142561.1
Coordinates 3984999..3985286 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS19540 (3981079) 3981079..3981327 + 249 WP_020325243.1 DUF1158 domain-containing protein -
KHV96_RS19545 (3981345) 3981345..3981686 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
KHV96_RS19550 (3981717) 3981717..3982832 - 1116 WP_020325248.1 MBL fold metallo-hydrolase -
KHV96_RS19555 (3983012) 3983012..3983593 + 582 WP_020325240.1 TetR/AcrR family transcriptional regulator -
KHV96_RS19560 (3983593) 3983593..3983961 + 369 WP_020325252.1 MmcQ/YjbR family DNA-binding protein -
KHV96_RS19565 (3984081) 3984081..3984734 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
KHV96_RS19570 (3984999) 3984999..3985286 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
KHV96_RS19575 (3985279) 3985279..3985596 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KHV96_RS19580 (3985781) 3985781..3986824 - 1044 WP_020325241.1 DUF2157 domain-containing protein -
KHV96_RS19585 (3987494) 3987494..3988360 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
KHV96_RS19590 (3988469) 3988469..3989896 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T202269 WP_004142563.1 NZ_CP074528:c3985596-3985279 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T202269 NZ_CP099364:696174-696515 [Citrobacter braakii]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTAGCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCACCTTATCTTCGGGCAGCAGATATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT202269 WP_004142561.1 NZ_CP074528:c3985286-3984999 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT202269 NZ_CP099364:695836-696153 [Citrobacter braakii]
ATGACCCACATCAACTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGACT
GCACTATCTGGCTGACAGGGCCAGCATCACCGGCAGGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATGCCCGC
ACTTTATCCGCCAGATGGAGTCGATGCTAACCACCGGTGAACTCAGTCCCCAACACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCTGATACTCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGAGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References