Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 323141..323727 Replicon chromosome
Accession NZ_CP074528
Organism Klebsiella pneumoniae strain S14_CRE14

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KHV96_RS01505 Protein ID WP_002920800.1
Coordinates 323359..323727 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KHV96_RS01500 Protein ID WP_004174006.1
Coordinates 323141..323362 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV96_RS01480 (319298) 319298..320224 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KHV96_RS01485 (320221) 320221..321498 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
KHV96_RS01490 (321495) 321495..322262 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KHV96_RS01495 (322264) 322264..322977 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KHV96_RS01500 (323141) 323141..323362 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KHV96_RS01505 (323359) 323359..323727 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KHV96_RS01510 (324000) 324000..325316 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KHV96_RS01515 (325423) 325423..326310 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KHV96_RS01520 (326307) 326307..327152 + 846 WP_004181473.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KHV96_RS01525 (327154) 327154..328224 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 320221..328961 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T202257 WP_002920800.1 NZ_CP074528:323359-323727 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T202257 NZ_CP099358:c2804459-2804208 [Escherichia fergusonii]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAACGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAAGAGACGGCTTATC
TATTGCGTTCCCCCGCTAATGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT202257 WP_004174006.1 NZ_CP074528:323141-323362 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT202257 NZ_CP099358:c2804211-2803957 [Escherichia fergusonii]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACTCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References