Detailed information of TA system TA202235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 68611..69338
• Replicon: plasmid pCRE10_1
• Accession: NZ_CP074520
• Organism: Klebsiella pneumoniae strain S10_CRE10

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: KHV94_RS25580
• Protein ID: WP_011251285.1
• Coordinates: 68611..68922 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KHV94_RS25585
• Protein ID: WP_011251286.1
• Coordinates: 68919..69338 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHV94_RS25550 (KHV94_25495)	63801..64781	+	981	WP_012569499.1	IS5-like element ISKpn26 family transposase	-
KHV94_RS25555 (KHV94_25500)	65328..65507	-	180	Protein_70	IS5/IS1182 family transposase	-
KHV94_RS25560 (KHV94_25505)	65547..67085	-	1539	WP_032430751.1	IS66 family transposase	-
KHV94_RS25565 (KHV94_25510)	67134..67481	-	348	WP_032430752.1	IS66 family insertion sequence element accessory protein TnpB	-
KHV94_RS25570 (KHV94_25515)	67478..67855	-	378	WP_004118218.1	transposase	-
KHV94_RS25575 (KHV94_25520)	67969..68406	+	438	Protein_74	DDE-type integrase/transposase/recombinase	-
KHV94_RS25580 (KHV94_25525)	68611..68922	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
KHV94_RS25585 (KHV94_25530)	68919..69338	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
KHV94_RS25590 (KHV94_25535)	69408..69821	+	414	WP_017901337.1	hypothetical protein	-
KHV94_RS25595 (KHV94_25540)	69867..70834	-	968	Protein_78	IS5-like element IS903B family transposase	-
KHV94_RS25600 (KHV94_25545)	70938..71204	+	267	WP_017900868.1	hypothetical protein	-
KHV94_RS25605 (KHV94_25550)	71318..71853	-	536	Protein_80	transposase	-
KHV94_RS25610 (KHV94_25555)	72378..73745	-	1368	WP_017900870.1	formimidoylglutamate deiminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..226793	226793
	-	inside	IScluster/Tn	-	-	63801..71536	7735

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T202235 WP_011251285.1 NZ_CP074520:68611-68922 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T202235 NZ_CP099350:251190-251471 [Escherichia fergusonii]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT202235 WP_011251286.1 NZ_CP074520:68919-69338 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT202235 NZ_CP099350:250949-251200 [Escherichia fergusonii]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1